Parasitology Research

, Volume 99, Issue 4, pp 341–345 | Cite as

Dynamics of Diplostomum spathaceum infection in snail hosts at a fish farm

  • Anssi Karvonen
  • Miia Savolainen
  • Otto Seppälä
  • E. Tellervo Valtonen
Original Paper


Ecologically sustainable disease prevention in intensive monocultures, such as fish farming, is based on the knowledge of the ecology of parasites and the identification of the key proportion of host populations for parasite life cycles. In this paper, we examined the life cycle dynamics of the pathogenic trematode Diplostomum spathaceum at a fish farm during a period of 1 year, focusing especially on the pattern of infection in the snail host (Lymnaea stagnalis) population, which is the key phase in the parasite life cycle regarding preventative actions. We found that (1) the infection varied seasonally in snails, being highest in late August; (2) the prevalence of infection and the proportion of individuals releasing cercariae were strongly dependent on snail size; (3) the parasite over-wintered in snails as dormant sporocysts and 4) the mortality of infected snails was not likely to differ from uninfected individuals during winter. Furthermore, the seasonality and development of the infection in the snails corresponded to the pattern of infection observed in the tracer rainbow trout caged in the farm area. This pattern of infection also indicated strong spatiality of the infection, probably because of differences in the size of the snail population between the ponds rather than differences in the prevalence of infection. Overall, present results indicate a possibility for late-summer outbreaks of D. spathaceum, but also that snail prevention at farms could be conducted more efficiently by considering both size-dependent infection and the timing of cercarial release.


  1. Bauer ON (1961) Parasitic diseases of cultured fishes and methods of their prevention and treatment. In: Dogiel VA, Petrushevski GK, Polyanski YI (eds) Parasitology of fishes. Oliver & Boyd, Edinburgh, pp 265–298Google Scholar
  2. Briers RA (2003) Range limits and parasite prevalence in a freshwater snail. Proc R Soc Lond B 270:S178–S180CrossRefGoogle Scholar
  3. Brown KM, Leathers BK, Minchella DJ (1988) Trematode prevalence and the population dynamics of freshwater pond snails. Am Midl Nat 120:289–301CrossRefGoogle Scholar
  4. Buchmann K, Uldal A (1994) Effects of eyefluke infections on the growth of rainbow trout (Oncorhynchus mykiss) in a mariculture system. Bull Eur Assoc Fish Pathol 14:104–107Google Scholar
  5. Buchmann K, Møller SH, Uldal A, Bresciani J (1997) Different seasonal infection dynamics of two species of eye-flukes (Diplostomum spathaceum and Tylodelphys clavata) in rainbow trout (Oncorhynchus mykiss). Bull Eur Assoc Fish Pathol 17:165–170Google Scholar
  6. Chappell LH, Hardie LJ, Secombes CJ (1994) Diplostomiasis: the disease and host-parasite interactions. In: Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara, Dyfed, pp 59–86Google Scholar
  7. Crowden AE, Broom DM (1980) Effects of the eyefluke, Diplostomum spathaceum, on the behaviour of dace (Leuciscus leuciscus). Anim Behav 28:287–294CrossRefGoogle Scholar
  8. Field JS, Irwin SWB (1994) The epidemiology, treatment and control of diplostomiasis on a fish farm in Northern Ireland. In: Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara, Dyfed, pp 87–100Google Scholar
  9. Goater TM, Shostak AW, Williams JA, Esch GW (1989) A mark-recapture study of trematode parasitism in overwintered Helisoma anceps (Pulmonata), with special reference to Halipegus occidualis (Hemiuridae). J Parasitol 75:553–560CrossRefGoogle Scholar
  10. Hakalahti T, Valtonen ET (2003) Population structure and recruitment of the ectoparasite Argulus coregoni Thorell (Crustacea: Branchiura) on a fish farm. Parasitology 127:79–85CrossRefPubMedGoogle Scholar
  11. Hudson PJ, Rizzoli A, Grenfell BT, Heesterbeek H, Dobson AP (2002) The ecology of wildlife diseases. Oxford University Press, OxfordGoogle Scholar
  12. Jokela J, Lively CM (1995) Spatial variation in infection by digenetic trematodes in a population of freshwater snails (Potamopyrgus antipodarum). Oecologia 103:509–517CrossRefGoogle Scholar
  13. Jokela J, Lively CM, Taskinen J, Peters AD (1999) Effect of starvation on parasite-induced mortality in a freshwater snail (Potamopyrgus antipodarum). Oecologia 119:320–325CrossRefGoogle Scholar
  14. Jokela J, Taskinen J, Mutikainen P, Kopp K (2005) Virulence of parasites in hosts under environmental stress: experiments with anoxia and starvation. Oikos 108:156–164CrossRefGoogle Scholar
  15. Karvonen A, Kirsi S, Hudson PJ, Valtonen ET (2004a) Patterns of cercarial production from Diplostomum spathaceum: terminal investment or bet hedging? Parasitology 129:87–92CrossRefPubMedGoogle Scholar
  16. Karvonen A, Seppälä O, Valtonen ET (2004b) Eye fluke-induced cataract formation in fish: quantitative analysis using an ophthalmological microscope. Parasitology 129:473–478CrossRefPubMedGoogle Scholar
  17. Karvonen A, Seppälä O, Valtonen ET (2004c) Parasite resistance and avoidance behaviour in preventing eye fluke infections in fish. Parasitology 129:159–164CrossRefPubMedGoogle Scholar
  18. Karvonen A, Paukku S, Seppälä O, Valtonen ET (2005) Resistance against eye flukes: naïve versus previously infected fish. Parasitol Res 95:55–59CrossRefPubMedGoogle Scholar
  19. Krist AC, Jokela J, Wiehn J, Lively CM (2004) Effects of host condition on susceptibility to infection, parasite developmental rate, and parasite transmission in a snail–trematode interaction. J Evol Biol 17:33–40CrossRefPubMedGoogle Scholar
  20. Lauckner G (1986) Ecological effects of larval trematode infestation on littoral marine invertebrate populations. In: Howell MJ (ed) Parasitology—quo vadit? Proceedings of the 6th international congress of parasitology, Brisbane, pp 391–397Google Scholar
  21. Loy C, Haas W (2001) Prevalence of cercariae from Lymnaea stagnalis snails in a pond system in southern Germany. Parasitol Res 87:878–882CrossRefPubMedGoogle Scholar
  22. McKeown CA, Irwin SWB (1997) Accumulation of Diplostomum spp. (Digenea: Diplostomatidae) metacercariae in the eyes of 0+ and 1+ roach (Rutilus rutilus). Int J Parasitol 27:377–380CrossRefPubMedGoogle Scholar
  23. Mo TA (1994) Status of Gyrodactylus salaris problems and research in Norway. Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara, Dyfed, pp 43–56Google Scholar
  24. Niewiadomska K (1986) Verification of the life-cycles of Diplostomum spathaceum (Rudolphi, 1819) and D. pseudospathaceum Niewiadomska, 1984 (Trematoda, Diplostomidae). Syst Parasitol 8:23–31Google Scholar
  25. Owen SF, Barber I, Hart PJB (1993) Low level infection by eye fluke, Diplostomum spp., affects the vision of three-spined sticklebacks, Gasterosteus aculeatus. J Fish Biol 42:803–806CrossRefGoogle Scholar
  26. Rintamäki-Kinnunen P, Valtonen ET (1996) Gyrodactylus salaris at fish farms in northern Finland over a period of ten years. Int J Parasitol 26:723–732CrossRefPubMedGoogle Scholar
  27. Rintamäki-Kinnunen P, Valtonen ET (1997) Epizootiology of protozoans in farmed salmonids at northern latitudes. Int J Parasitol 27:89–99CrossRefPubMedGoogle Scholar
  28. Roth M, Richards RH, Sommerville C (1993) Current practises in the chemotherapeutic control of sea lice infestations in aquaculture: a review. J Fish Dis 16:1–26CrossRefGoogle Scholar
  29. Sangster CR, Dove ADM, Bowser PR (2004) Diplostomum in pond-reared walleye Stizostedion vitreum—implications of a management strategy for control. Aquaculture 236:95–102CrossRefGoogle Scholar
  30. Seppälä O, Karvonen A, Valtonen ET (2004) Parasite-induced change in host behaviour and susceptibility to predation in an eye fluke–fish interaction. Anim Behav 68:257–263CrossRefGoogle Scholar
  31. Shariff M, Richards RH, Sommerville C (1980) The histopathology of acute and chronic infections of rainbow trout Salmo gairdneri Richardson with eye flukes, Diplostomum spp. J Fish Dis 3:455–465CrossRefGoogle Scholar
  32. Stables JN, Chappell LH (1986) The epidemiology of diplostomiasis in farmed rainbow trout from north-east Scotland. Parasitology 92:699–710PubMedGoogle Scholar
  33. Sweeting RA (1974) Investigations into natural and experimental infections of freshwater fish by the common eye-fluke Diplostomum spathaceum Rud. Parasitology 69:291–300PubMedCrossRefGoogle Scholar
  34. Valtonen ET, Gibson DI (1997) Aspects of the biology of diplostomid metacercarial (Digenea) populations occurring in fishes in different localities in northern Finland. Ann Zool Fenn 34:47–59Google Scholar
  35. Valtonen ET, Holmes JC, Koskivaara M (1997) Eutrophication, pollution and fragmentation: effects on parasite communities of roach (Rutilus rutilus) and perch (Perca fluviatilis) in four lakes in central Finland. Can J Fish Aquat Sci 54:572–585CrossRefGoogle Scholar
  36. Valtonen ET, Holmes JC, Aronen J, Rautalahti I (2003) Parasite communities as indicators of recovery from pollution: parasites of roach (Rutilus rutilus) and perch (Perca fluviatilis) in Central Finland. Parasitology 126:S43–S52CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Anssi Karvonen
    • 1
  • Miia Savolainen
    • 1
  • Otto Seppälä
    • 1
  • E. Tellervo Valtonen
    • 1
  1. 1.Department of Biological and Environmental ScienceUniversity of JyväskyläJyväskyläFinland

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