Occurrence and molecular characterization of Cryptosporidium spp. in mammals and reptiles at the Lisbon Zoo
- 326 Downloads
The presence of Cryptosporidium parasites in mammals and reptiles kept at the Lisbon Zoo was investigated. A total of 274 stool samples were collected from 100 mammals and 29 reptiles. The species and genotype of the isolates identified by light microscopy were determined by nested PCR and sequence analysis of a fragment of the small subunit rRNA gene. Cryptosporidium oocysts were found in one black wildebeest (Connochaetes gnou), one Prairie bison (Bison bison bison) and in one Indian star tortoise (Geochelone elegans). The PCR and sequence analysis of these three isolates showed that those excreted by the Prairie bison were Cryptosporidium mouse genotype, those from the black wildebeest were from a new Cryptosporidium genotype and those infecting the Indian star tortoise were Cryptosporidium tortoise genotype. The present work reports a new Cryptosporidium genotype in a black wildebeest and the first finding of the Cryptosporidium mouse genotype in a ruminant.
KeywordsCryptosporidiosis Cryptosporidium Oocyst Cryptosporidium Infection Mouse Genotype Bison Bison
This work was supported by the projects POCTI/ESP/43635/99 and POCTI/ESP/46369/2002 from the Fundação para a Ciência e Tecnologia (FCT). Margarida Alves received a PhD thesis grant from FCT (SFRH/BD/2898/200). The authors would like to thank the workers of the Lisbon Zoo for their cooperation in sample collection.
- Alves M, Matos O, Fonseca IP, Delgado E, Lourenço AM, Antunes F (2001) Multilocus genotyping of Cryptosporidium isolates from human HIV-infected and animal hosts. J Euk Microbiol (Suppl):17S-18SGoogle Scholar
- Graczyk TK, Balazs GH, Work T, Aguirre AA, Ellis DM, Murakawa SKK, Morris R (1997) Cryptosporidium sp. infections in green turtles, Chelonia mydas, as a potential source of marine waterborne occysts in the Hawaiian Islands. Appl Environ Microbiol 63:2925–2927Google Scholar
- Majewska AC, Kasprzak W, Werner A (1997) Prevalence of Cryptosporidium in mammals housed in Poznań Zoological Garden, Poland. Acta Parasitol 42:195–198Google Scholar
- Morgan UM, Strudee AP, Singleton G, Gómez MS, Gracenea M, Torres J, Hamilton SG, Woodside DP, Thompson RCA (1999b) The Cryptosporidium “mouse” genotype is conserved across geographic areas. J Clin Microbiol 37:1302–1305Google Scholar