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Parasitology Research

, Volume 97, Issue 2, pp 108–112 | Cite as

Occurrence and molecular characterization of Cryptosporidium spp. in mammals and reptiles at the Lisbon Zoo

  • Margarida Alves
  • Lihua Xiao
  • Vanessa Lemos
  • Ling Zhou
  • Vitaliano Cama
  • Margarida Barão da Cunha
  • Olga MatosEmail author
  • Francisco Antunes
Original Paper

Abstract

The presence of Cryptosporidium parasites in mammals and reptiles kept at the Lisbon Zoo was investigated. A total of 274 stool samples were collected from 100 mammals and 29 reptiles. The species and genotype of the isolates identified by light microscopy were determined by nested PCR and sequence analysis of a fragment of the small subunit rRNA gene. Cryptosporidium oocysts were found in one black wildebeest (Connochaetes gnou), one Prairie bison (Bison bison bison) and in one Indian star tortoise (Geochelone elegans). The PCR and sequence analysis of these three isolates showed that those excreted by the Prairie bison were Cryptosporidium mouse genotype, those from the black wildebeest were from a new Cryptosporidium genotype and those infecting the Indian star tortoise were Cryptosporidium tortoise genotype. The present work reports a new Cryptosporidium genotype in a black wildebeest and the first finding of the Cryptosporidium mouse genotype in a ruminant.

Keywords

Cryptosporidiosis Cryptosporidium Oocyst Cryptosporidium Infection Mouse Genotype Bison Bison 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by the projects POCTI/ESP/43635/99 and POCTI/ESP/46369/2002 from the Fundação para a Ciência e Tecnologia (FCT). Margarida Alves received a PhD thesis grant from FCT (SFRH/BD/2898/200). The authors would like to thank the workers of the Lisbon Zoo for their cooperation in sample collection.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Margarida Alves
    • 1
  • Lihua Xiao
    • 2
  • Vanessa Lemos
    • 1
  • Ling Zhou
    • 2
  • Vitaliano Cama
    • 2
  • Margarida Barão da Cunha
    • 3
  • Olga Matos
    • 1
    Email author
  • Francisco Antunes
    • 1
    • 4
  1. 1.Unidade de Protozoàrios Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Médicas (UPMM), Instituto de Higiene e Medicina TropicalUniversidade Nova de LisboaLisboaPortugal
  2. 2.Division of Parasitic DiseasesCenters for Disease Control and PreventionAtlantaUSA
  3. 3.Jardim Zoológico de LisboaEstrada de BenficaLisboaPortugal
  4. 4.Clínica Universitária de Doenças Infecciosas, Faculdade de Medicina (Hospital de Santa Maria)Universidade de LisboaLisboaPortugal

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