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Parasitology Research

, Volume 96, Issue 5, pp 321–325 | Cite as

Genetic variations of the dihydrofolate reductase gene of Plasmodium vivax in Mandalay Division, Myanmar

  • Byoung-Kuk Na
  • Hyeong-Woo Lee
  • Sung-Ung Moon
  • Tae-Suk In
  • Khin Lin
  • Maung Maung
  • Gyung-Tae Chung
  • Jong-Koo Lee
  • Tong-Soo Kim
  • Yoon Kong
Original Paper

Abstract

Dihydrofolate reductase (DHFR; EC1.5.1.3) is a known target enzyme for antifolate agents, which are used as alternative chemotherapeutics for chloroquine-resistant malaria. Mutations in the dhfr gene of Plasmodium vivax are thought to be associated with resistance to the antifolate drugs. In this study, we have analyzed genetic variations in the dhfr genes of clinical isolates of P. vivax (n=21) in Myanmar, to monitor antifolate resistance in this country. Sequence variations within the entire dhfr gene were highly restricted to codons from 57 to 117, and the GGDN tandem repeat region. Double (S58R and S117N/T) or quadruple mutations (F57L/I, S58R, T61M, and S117N/T), which may be closely related to the drug resistance, were recognized in most of the isolates (20/21 cases). Our results suggest that antifolate-resistant P. vivax is becoming widespread in Myanmar, as it also is in the neighboring countries in Southeast Asia. It appears that the drug resistance situation may be worsening in the country.

Keywords

Plasmodium vivax Dihydrofolate reductase Point mutations Myanmar 

Notes

Acknowledgements

This work was supported by a grant from the Korea Health 21 R&D Project, Ministry of Health and Welfare, the Republic of Korea (03-PJ1-PG1-CH01-0001). The authors are grateful to health personnel of Department of Health (Vector Borne Diseases Control Project and Basic Health in the respected townships) and Department of Medical Research (Upper Myanmar), for their active cooperation in this study.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Byoung-Kuk Na
    • 1
  • Hyeong-Woo Lee
    • 2
  • Sung-Ung Moon
    • 2
  • Tae-Suk In
    • 2
  • Khin Lin
    • 3
  • Maung Maung
    • 3
  • Gyung-Tae Chung
    • 2
  • Jong-Koo Lee
    • 4
  • Tong-Soo Kim
    • 2
  • Yoon Kong
    • 1
  1. 1.Department of Molecular Parasitology, Center for Molecular Medicine, Samsung Biomedical Research InstituteSungkyunkwan University School of MedicineSuwonKorea
  2. 2.Department of Tropical and Endemic Parasitic Diseases, National Institute of HealthKorea Center for Disease Control and PreventionSeoulKorea
  3. 3.Department of Medical Research (Upper Myanmar)Pying Oo Lwin TownshipMandalayMyanmar
  4. 4.Bureau of Health PromotionMinistry of Health and WelfareGwacheonKorea

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