In vitro stimulation of penetration gland emptying by Trichobilharzia szidati and T. regenti (Schistosomatidae) cercariae. Quantitative collection and partial characterization of the products
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Induction of penetration gland emptying by cercariae of the bird schistosomes Trichobilharzia szidati and T. regenti employing linoleic acid, linolenic acid, praziquantel and calcium ionophore A23187 showed that both postacetabular and circumacetabular cells released their content at chosen stimulant concentrations. The gland secretions consisted of soluble and insoluble parts. The former one adhering to the ground seemed to have different saccharide composition from the glands of Schistosoma mansoni. It bound labelled saccharides, thus exhibiting lectin-like activity. Protein profiles of the latter one were identical after stimulation by all four stimulants in T. szidati. The soluble secretions contained several proteolytic enzymes; 31 kDa and 33 kDa cysteine proteases were identified in E/S products of T. szidati and T. regenti, respectively. The circumacetabular glands contained a significant amount of calcium. Immunohistochemistry revealed that the origin of E/S products after in vitro stimulation is in both penetration glands and tegumental structures. No crossreactivity was observed between the bird schistosomes and a serum raised against S. mansoni elastase.
KeywordsPraziquantel Gland Secretion Calcium Ionophore A23187 Ligand Blotting Penetration Gland
We wish to thank Prof. M. Doenhoff, University of Wales, Bangor, UK and Dr. A. Mountford, University of York, UK, for provision of S. mansoni cercariae, E/S products and antielastase antibodies. Our grateful thanks belong also to Dr. C. Caffrey and Dr. D. Greenbaum, Sandler Center for Basic Research in Parasitic Diseases, UCSF, USA, for the supply of DCG-04 cysteine protease probe, and Prof. M. Tichá, Department of Biochemistry, Charles University Prague, for biotinylated glycosaminoglycans. The project was supported by the grants of the Grant Agency of the Czech Republic no. 524/04/P082 and 524/03/1263, Grant Agency of the Charles University no. 263/2004/B/Bio, the Wellcome Trust Collaborative Research Initiative Grant no. 072255 and the grant of the Ministry of Education of the Czech Republic no. 0021620828. The experiments presented in this paper comply with current laws of the Czech Republic.
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