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Parasitology Research

, Volume 96, Issue 1, pp 6–11 | Cite as

Experimental schistosomiasis, protective aspects of granulomatous reaction in the mouse liver

  • Stéphane Hanna
  • Bouchra Gharib
  • Hubert Lepidi
  • Jean-Claude Montet
  • Henri Dumon
  • Max de ReggiEmail author
Original Paper

Abstract

We show two mechanisms of liver protection by the granulomatous reaction against Schistosoma mansoni eggs entrapped in the organ. First, eosinophil peroxidase and its substrate H2O2 are released by inflammatory cells in the immediate vicinity of the parasite eggs. The efficiency of this process was demonstrated by administration of antioxidants to infected mice. The treatment, which reduces H2O2 production, significantly improved the ability of parasite eggs to hatch after collection from the liver. Secondly, we labeled the released egg antigens in liver histological sections and we found that the lattice of collagen fibers which is built around eggs appears to create a barrier preventing released compounds from diffusing freely in surrounding tissues. Together, oxidative processes and antigen containment allow the parasitized liver to cope with the dual threat posed by parasite eggs, i.e. a highly resistant chitinous eggshell and the release of toxic substances.

Keywords

Infected Mouse Schistosomiasis H2O2 Production Hamster Liver H2DCF Oxidization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors are indebted to Howard Rickenberg (Immunotech, Marseille, France) and Robert Loubière (Laboratoire d’Anatomie Pathologique, Nice, France) for stimulating discussions and for helping with the manuscript. The authors declare that the experiments comply with current French laws.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Stéphane Hanna
    • 1
  • Bouchra Gharib
    • 1
  • Hubert Lepidi
    • 2
  • Jean-Claude Montet
    • 3
  • Henri Dumon
    • 1
  • Max de Reggi
    • 1
    Email author
  1. 1.INSERM U399, IFR 48Univ. MediterraneeMarseilleFrance
  2. 2.CNRS-UMR 6020, IFR 48Univ. MediterraneeMarseilleFrance
  3. 3.UPRES-EA 3286, IFR 48Univ. MediterraneeMarseilleFrance

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