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Parasitology Research

, Volume 95, Issue 4, pp 273–277 | Cite as

Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources

  • Jacob Lorenzo-Morales
  • Antonio Ortega-RivasEmail author
  • Pilar Foronda
  • Enrique Martínez
  • Basilio Valladares
Original Paper

Abstract

A comprehensive survey to document the presence of free-living amoebae of the genus Acanthamoeba was conducted in tap water and sea water sources related to human environments in Tenerife, Canary Islands, Spain. Acanthamoeba identification was based on the morphology of cyst and trophozoite forms and PCR amplification with a genus-specific primer pair. The pathogenic potential of Acanthamoeba isolates was characterized by temperature and osmotolerance assays and PCR reactions with two primer pairs related to Acanthamoeba pathogenesis. The results demonstrate the presence of potentially pathogenic strains in both sources. Thus, some of the amoebae in these aquatic habitats can act as opportunistic pathogens, could play a role in the diseases of aquatic organisms, and may present a risk to human health.

Keywords

Keratitis Canary Island Acanthamoeba Isolate Acanthamoeba Strain Species Specific Prime Pair 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

This work was supported by Dirección General de Universidades del Gobierno de Canarias.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Jacob Lorenzo-Morales
    • 1
  • Antonio Ortega-Rivas
    • 1
    Email author
  • Pilar Foronda
    • 1
  • Enrique Martínez
    • 1
  • Basilio Valladares
    • 1
  1. 1.Department of ParasitologyUniversity of La LagunaLa LagunaSpain

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