Advertisement

Parasitology Research

, Volume 99, Issue 1, pp 37–44 | Cite as

Ultrastructure of the spermatozoon of Dollfusiella spinulifera (Beveridge and Jones, 2000) Beveridge, Neifar and Euzet, 2004 (Trypanorhyncha, Eutetrarhynchidae)

  • Jordi MiquelEmail author
  • Zdzisław Świderski
Original Paper

Abstract

The ultrastructural organization of the mature spermatozoon of the trypanorhynch cestode Dollfusiella spinulifera is described. The spermatozoon is a long filiform cell, tapered at both ends, which lacks mitochondria. Its cytoplasm contains (1) two axonemes of different lengths of the 9+‘1’ pattern of trepaxonematan Platyhelminthes, (2) two rows of parallel cortical microtubules, each of which is adjacent to the opposite sides of the plasma membrane, (3) the nucleus, and (4) glycogen in the form of both α-glycogen rosettes and β-glycogen particles. Unlike the majority of cestode spermatozoa, the spematozoon of D. spinulifera lacks crested bodies and periaxonemal sheath. In view of the present results, the postulated synapomorphic value of crested bodies for the Eucestoda is questioned.

Keywords

Sodium Cacodylate Buffer Mature Spermatozoon Cortical Microtubule Posterior Extremity Anterior Extremity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

Authors wish to thank Dr. Malcolm K. Jones (Centre for Microscopy and Microanalysis, University of Queensland, Australia) for providing the embedded specimens of D. spinulifera and Dr. Ian Beveridge (Department of Veterinary Science, University of Melbourne, Australia) for confirming the identification of these specimens. We also wish to thank the “Serveis Cientificotècnics” (University of Barcelona, Spain) for their support in the preparation of samples. This study was supported by the “Departament d’Universitats, Recerca i Societat de la Informació, Generalitat de Catalunya” (Spain).

References

  1. Azzouz-Draoui N, Mokhtar-Maamouri F (1986–1988) Ultrastructure comparée de la spermiogenèse et du spermatozoïde de Echinobothrium affine Diesing, 1863 et E. harfordi Mac Vicar, 1976 (Cestoda, Diphyllidea). Bull Soc Sci Nat Tunis 18:9–20Google Scholar
  2. Bâ CT, Marchand B (1994) Ultrastructure of the spermatozoon of Sandonella sandoni (Cestoda, Proteocephalidea, Sandonellinae). Invertebr Reprod Dev 25:9–17Google Scholar
  3. Bâ CT, Marchand B (1995) Spermiogenesis, spermatozoa and phyletic affinities in the Cestoda. In: Jamieson BGM, Ausió J, Justine JL (eds) Advances in spermatozoal phylogeny and taxonomy. Mem Mus Natl Hist Nat 166:87–95Google Scholar
  4. Bâ A, Bâ CT, Marchand B (2002) Ultrastructural study of the spermatozoon of Echinocotyle dolosa (Cestoda, Cyclophyllidea, Hymenolepididae). Acta Parasitol 47:131–136Google Scholar
  5. Bâ CT, Bâ A, Marchand B (2005) Ultrastructure of the spermatozoon of Raillietina (Raillietina) baeri (Cyclophyllidea, Davaineidae) an intestinal parasite of the multimammate rat, Mastomys huberti (Rodentia, Muridae). Parasitol Res 97:173–178CrossRefPubMedGoogle Scholar
  6. Bruňanská M, Nebesářová J, Scholz T, Fagerholm HP (2002) Ultrastructure of the spermatozoon of the pseudophyllidean cestode Eubothrium crassum (Bloch, 1779). Parasitol Res 88:285–291CrossRefPubMedGoogle Scholar
  7. Bruňanská M, Scholz T, Nebesářová J (2003a) Reinvestigation of the spermatozoon ultrastructure of the cestode Proteocephalus longicollis (Zeder, 1800), a parasite of salmonid fish. Parasitol Res 91:357–362CrossRefPubMedGoogle Scholar
  8. Bruňanská M, Nebesářová J, Scholz T (2003b) Ultrastructure of the spermatozoon of the proteocephalidean cestode Proteocephalus torulosus (Batsch, 1786). Parasitol Res 89:345–351PubMedGoogle Scholar
  9. Bruňanská M, Scholz T, Ibraheem MH (2004a) Ultrastructural characters of the spermatozoon of the cestode Corallobothrium solidum Fritsch, 1886 (Cestoda: Proteocephalidea), a parasite of the electric catfish Malapterurus electricus. Parasitol Res 94:421–426CrossRefPubMedGoogle Scholar
  10. Bruňanská M, Scholz T, Ibraheem MH (2004b) Ultrastructural particularities of the spermatozoon of the cestode Electrotaenia malopteruri (Fritsch, 1886) (Proteocephalidae: Gangesiinae), a parasite of Malapterurus electricus (Siluriformes: Malapteruridae) from the river Nile, Egypt. Parasitol Res 93:114–120CrossRefPubMedGoogle Scholar
  11. Campbell RA, Beveridge I (1994) Order Trypanorhyncha Diesing, 1863. In: Khalil LF, Jones A, Bray RA (eds) Keys to the cestode parasites of vertebrates. CAB International, Wallingford, pp 51–148Google Scholar
  12. Ehlers U (1985) Phylogenetic relationships within the Platyhelminthes. In: Conway Morris S, George JD, Gibson R, Platt HM (eds) The origins and relationships of lower invertebrates. Oxford University Press, OxfordGoogle Scholar
  13. Euzet L, Świderski Z, Mokhtar-Maamouri F (1981) Ultrastructure comparée du spermatozoide des cestodes. Relations avec la phylogénèse. Ann Parasitol (Paris) 56:247–259Google Scholar
  14. Hidalgo C, Miquel J, Torres J, Marchand B (2000) Ultrastructural study of spermiogenesis and the spermatozoon in Catenotaenia pusilla, an intestinal parasite of Mus musculus. J Helminthol 74:73–81PubMedGoogle Scholar
  15. Hoberg EP, Gardner SL, Campbell RA (1997) Paradigm shifts and tapeworm systematics. Parasitol Today 13:161–162CrossRefGoogle Scholar
  16. Hoberg EP, Gardner SL, Campbell RA (1999) Systematics of the Eucestoda: advances toward a new phylogenetic paradigm, and observations on the early diversification of tapeworms and vertebrates. Syst Parasitol 42:1–12CrossRefPubMedGoogle Scholar
  17. Justine J-L (1986) Ultrastructure of the spermatozoon of the cestode Duthiersia fimbriata (Pseudophyllidea, Diphyllobothriidae). Can J Zool 64:1545-1548CrossRefGoogle Scholar
  18. Justine J-L (1991) Phylogeny of parasitic Platyhelminthes: a critical study of synapomorphies proposed on the basis of the ultrastructure of spermiogenesis and spermatozoa. Can J Zool 69:1421–1440Google Scholar
  19. Justine J-L (1998) Spermatozoa as phylogenetic characters for the Eucestoda. J Parasitol 84:385–408PubMedGoogle Scholar
  20. Justine J-L (2001) Spermatozoa as phylogenetic characters for the Platyhelminthes. In: Littlewood DTJ, Bray RA (eds) Interrelationships of the Platyhelminthes. Taylor and Francis, London, pp 231–238Google Scholar
  21. Khalil LF, Jones A, Bray RA (1994) Keys to the cestode parasites of vertebrates. CAB International, Wallingford, p 751Google Scholar
  22. Levron C, Bruňanská M, Marchand B (2005) Spermiogenesis and sperm ultrastructure of the pseudophyllidean cestode Triaenophorus nodulosus (Pallas, 1781). Parasitol Res 98:26–33Google Scholar
  23. MacKinnon BM, Burt MDB (1984) The comparative ultrastructure of spermatozoa from Bothrimonus sturionis Duv. 1842 (Pseudophyllidea), Pseudanthobothrium hanseni Baer, 1956 (Tetraphyllidea), and Monoecocestus americanus Stiles, 1895 (Cyclophyllidea). Can J Zool 62:1059–1066CrossRefGoogle Scholar
  24. MacKinnon BM, Burt MDB (1985) Ultrastructure of spermatogenesis and the mature spermatozoon of Haplobothrium globuliforme Cooper, 1914 (Cestoda: Haplobothrioidea). Can J Zool 63:1478–1487Google Scholar
  25. Mahendrasingam S, Fairweather I, Halton DW (1989) Spermatogenesis and the fine structure of the mature spermatozoon in the free proglottis of Trilocularia acanthiaevulgaris (Cestoda, Tetraphyllidea). Parasitol Res 75:287–298CrossRefPubMedGoogle Scholar
  26. McKerr G (1985) The fine structure and physiology of a trypanorhynch tapeworm Grillotia erinaceus. Ph.D. Thesis, The Queen’s University of BelfastGoogle Scholar
  27. Miquel J, Feliu C, Marchand B (1999) Ultrastructure of spermiogenesis and the spermatozoon of Mesocestoides litteratus (Cestoda, Mesocestoididae). Int J Parasitol 29:499–510PubMedGoogle Scholar
  28. Miquel J, Świderski Z, Młocicki D, Marchand B (2004) Ultrastructure of the spermatozoon of the anoplocephalid cestode Gallegoides arfaai (Mobedi and Ghadirian, 1977) Tenora and Mas-Coma, 1978, an intestinal parasite of the wood mouse (Apodemus sylvaticus Linnaeus, 1758). Parasitol Res 94:460–467CrossRefPubMedGoogle Scholar
  29. Miquel J, Świderski Z, Marchand B (2005) Spermatological characters in the Dipylidiidae Stiles, 1896 (Cestoda, Cyclophyllidea). Acta Parasitol 50:65–73Google Scholar
  30. Mokhtar-Maamouri F (1979) Étude en microscopie électronique de la spermiogenèse et du spermatozoïde de Phyllobothrium gracile Weld, 1855 (Cestoda, Tetraphyllidea, Phyllobothriidae). Z Parasitenkd 59:245–258CrossRefGoogle Scholar
  31. Mokhtar-Maamouri F (1982) Étude ultrastructurale de la spermiogenèse de Acanthobothrium filicolle var. filicolle Zschokke, 1888 (Cestoda, Tetraphyllidea, Onchobothriidae). Ann Parasitol (Paris) 57:429–442Google Scholar
  32. Mokhtar-Maamouri F, Azzouz-Draoui N (1990) Spermiogenèse et ultrastructure du spermatozoïde de Nematotaenia chantalae Dollfus, 1957 (Cestoda, Cyclophyllidea, Nematotaeniidae). Ann Parasitol Hum Comp 65:221–228Google Scholar
  33. Mokhtar-Maamouri F, Świderski Z (1975) Étude en microscopie électronique de la spermatogénèse de deux Cestodes Acanthobothrium filicolle benedenii Loennberg, 1889 et Onchobothrium uncinatum (Rud., 1819) (Tetraphyllidea, Onchobothriidae). Z Parasitenkd 47:269–281CrossRefPubMedGoogle Scholar
  34. Ndiaye PI, Miquel J, Marchand B (2003) Ultrastructure of spermiogenesis and spermatozoa of Taenia parva Baer, 1926 (Cestoda, Cyclophyllidea, Taeniidae), a parasite of the common genet (Genetta genetta). Parasitol Res 89:34–43CrossRefPubMedGoogle Scholar
  35. Olson PD, Littlewood DTJ, Bray RA, Mariaux J (2001) Interrelationships and evolution of the tapeworms (Platyhelminthes: Cestoda). Mol Phylogenet Evol 19:443–467CrossRefPubMedGoogle Scholar
  36. Palm HW (2004) The Trypanorhyncha Diesing, 1863. PKSPL-IPB Press, Bogor, p 710Google Scholar
  37. Rohde K, Watson N (1986) Ultrastructure of spermatogenesis and sperm of Austramphilina elongata (Platyhelminthes, Amphilinidea). J Submicrosc Cytol 18:361–374Google Scholar
  38. Sène A, Bâ CT, Marchand B (1997) Ultrastructure of spermiogenesis and the spermatozoon of Nomimoscolex sp. (Cestoda, Proteocephalidea) intestinal parasite of Clarotes laticeps (Fish, Teleost) in Senegal. J Submicrosc Cytol Pathol 29:1–6PubMedGoogle Scholar
  39. Stoitsova SR, Georgiev BB, Dacheva RB (1995) Ultrastructure of spermiogenesis and the mature spermatozoon of Tetrabothrius erostris Loennberg, 1896 (Cestoda, Tetrabothriidae). Int J Parasitol 25:1427–1436CrossRefPubMedGoogle Scholar
  40. Świderski Z (1968) The fine structure of the spermatozoon of sheep tapeworm, Moniezia expansa (Rud., 1810) (Cyclophyllidea, Anoplocephalidae). Zool Pol 18:475–486Google Scholar
  41. Świderski Z (1976) Fine structure of the spermatozoon of Lacistorhynchus tenuis (Cestoda, Trypanorhyncha). Proceedings of the 6th European congress on electron microscopy, Jerusalem, pp 309–310Google Scholar
  42. Świderski Z (1994) Spermiogenesis in the Trypanorhynchid Cestode Lacistorhynchus tenuis. Proceedings of the 13th international congress on electron microscopy, Paris, pp 691–692Google Scholar
  43. Świderski Z, Mackiewicz JS (2002) Ultrastructure of spermatogenesis and spermatozoa of the caryophyllidean cestode Glaridacris catostomi Cooper, 1920. Acta Parasitol 47:83–104Google Scholar
  44. Świderski Z, Salamatin RV, Tkach VV (2000) Electron microscopial study of spermatozoa of the cestode Dilepis undula (Cyclophyllidea, Dilepididae). Vest Zool 34:3–7Google Scholar
  45. Weekes PJ (1987) Studies on the biology of some nippotaenid cestodes. Ph.D. Thesis, Victoria University of WellingtonGoogle Scholar
  46. Xylander WER (1989) Ultrastructural studies on the reproductive system of Gyrocotylidea and Amphilinidea (Cestoda): spermatogenesis, spermatozoa, testes and vas deferens of Gyrocotyle. Int J Parasitol 19:897–905Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Laboratori de Parasitologia, Departament de Microbiologia i Parasitologia Sanitàries, Facultat de FarmàciaUniversitat de BarcelonaBarcelonaSpain
  2. 2.W. Stefański Institute of ParasitologyPolish Academy of SciencesWarsawPoland
  3. 3.Department of General Biology and ParasitologyWarsaw Medical UniversityWarsawPoland

Personalised recommendations