Parasitology Research

, Volume 98, Issue 4, pp 349–354

Comparative analysis of Trichinella spiralis and Trichinella nativa proteins by two-dimensional gel electrophoresis

Original Paper

Abstract

Trichinella spiralis and Trichinella nativa are both common wildlife parasites in Finland. However, they differ substantially in their resistance to below 0°C temperatures in their natural hosts. T. nativa can live in frozen fox meat for years, whereas T. spiralis dies when frozen. In mouse muscle, the difference is not as evident; even T. nativa cannot maintain infectivity when kept at −20°C for 1 week. Crude larval protein extracts of these two parasite species were analyzed by two-dimensional gel electrophoresis (2DE). The protein patterns showed clear differences, but matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprint followed by database searches failed to identify these proteins, suggesting that they may still be uncharacterized. The patterns compared after freezing treatment at −20°C revealed changes in the intensity of some protein spots. The antigenic differences of the species were analyzed with two-dimensional Western blots, which showed T. spiralis-specific proteins.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • A. Näreaho
    • 1
  • K. Ravanko
    • 2
  • E. Hölttä
    • 2
  • A. Sukura
    • 1
  1. 1.Department of Basic Veterinary Sciences, Faculty of Veterinary MedicineUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Pathology, Haartman InstituteUniversity of HelsinkiHelsinkiFinland

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