The association of Blastocystis hominis and Endolimax nana with diarrheal stools in Zambian school-age children
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To determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the Southern Province of Zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n=93). Almost half of the stools (49.5%) were diarrhetic. The overall prevalence of Endolimax nana, Schistosoma haematobium, Blastocystis hominis, Giardia lamblia, Cryptosporidium parvum, Encephalitozoon intestinalis, and Strongyloides stercoralis was 64.3, 59.1, 53.8, 19.4, 8.6, 8.6, and 1.1%, respectively. Only the associations between infection with B. hominis and E. nana with diarrhea were statistically significant. Although B. hominis and E. nana are considered to be nonpathogenic organisms, this study demonstrated that they can be associated with diarrhea in children when they occur at high prevalence and intensity. This survey supports the recent evidence that B. hominis and E. nana infections are associated with deficient sanitation and low hygiene standards and can contribute to diarrhea in children in developing countries.
KeywordsIrritable Bowel Syndrome Chronic Diarrhea Schistosoma Haematobium Haematobium Infection Enterocytozoon Bieneusi
We thank Zambian health officials and employees of The Malaria Institute at Macha, Zambia, for facilitating this study. The study was supported by the Johns Hopkins Malaria Research Institute, Baltimore, MD, USA.
- Ash LR, Orihel TC (1987) Parasites: a guide to laboratory procedures and identification. American Society of Clinical Parasitologists, ChicagoGoogle Scholar
- Carrascosa M, Martinez J, Perez-Castrillon JL (1996) Hemorrhagic proctosigmoiditis and Blastocystis hominis infection. Ann Intern Med 15:278–279Google Scholar
- Curtis J, Sleisenger MH (1973) Infectious and parasitic diseases. In: Sleisenger MH, Fordtrain JS (eds) Gastrointestinal disease. WB Saunders, Philadelphia pp 1369–1405Google Scholar
- Healy GR, Garcia LS (1995) Intestinal and urogenital protozoa. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds) Manual of clinical microbiology. ASM Press, Washington DC, pp 1204–1228Google Scholar
- Swerdlow MA, Burrows RB (1955) Dientamoeba fragilis, an intestinal pathogen. JAMA 158:176–178Google Scholar
- WHO (1995) The treatment of diarrhea: a manual for physicians and senior health workers. Bull World Health Org/CDR/95.3:1–47Google Scholar
- WHO (1998). Persistent diarrhea in children in developing countries: memorandum from WHO meeting. Bull World Health Org 66:709–717Google Scholar
- Wolfe MS (2000) Miscellaneous intestinal protozoa. In: Strickland GT (ed) Hunter's tropical medicine and emerging infectious diseases. WB Saunders, Philadelphia, pp 603–610Google Scholar
- Zaman V (1996) Blastocystis hominis. In: Weatherall DJ, Ledingham JGG, Warrell D (eds) Oxford Texbook of Medicine 3rd edn. Oxford University Press, OxfordGoogle Scholar
- Zierdt CH (1991) Blastocystis hominis: past and future. Microbiol Rev 4:61–79Google Scholar