Parasitology Research

, Volume 95, Issue 4, pp 287–292

Identification of strongyle eggs from anthelmintic-treated horses using a PCR-ELISA based on intergenic DNA sequences

  • J. E. Hodgkinson
  • K. L. Freeman
  • J. R. Lichtenfels
  • S. Palfreman
  • S. Love
  • J. B. Matthews
Original Paper


The efficacy of five daily fenbendazole (FBZ) treatments was tested against benzimidazole-resistant cyathostomins in naturally infected horses (n=13). Horses were treated with pyrantel embonate (PYR) to remove adult strongyles followed, 7 days later, by a 5-day course of FBZ. The PYR treatment produced an average faecal egg count reduction of 98%. All samples were negative by faecal egg count 7 days after the start of the FBZ treatment. Positive egg counts were observed from 28 days after the start of FBZ treatment and all horses displayed positive faecal egg counts by 77 days after treatment. Strongyle eggs were harvested from the faeces of the horses prior to treatment and then weekly from 42 to 70 days post-treatment. DNA was obtained from eggs in groups of ten. A PCR-ELISA, based on species-specific differences in intergenic DNA sequences, was used to identify the presence of six cyathostomin species. In pre-treatment samples, Cyathostomum catinatum was detected in nine out of the 13 horses and Cylicostephanus longibursatus, Cylicostephanus goldi and Cylicocyclus nassatus, were found in samples from eight animals. Cylicocyclus ashworthi and Cylicocyclus insigne were not detected pre-treatment. After anthelmintic treatment, C. catinatum and C. longibursatus were most frequently detected, followed by C. nassatus, C. goldi and C. ashworthi. C. insigne was detected at only one time point in a sample from a single horse.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • J. E. Hodgkinson
    • 1
  • K. L. Freeman
    • 2
  • J. R. Lichtenfels
    • 3
  • S. Palfreman
    • 2
  • S. Love
    • 4
  • J. B. Matthews
    • 5
  1. 1.Department of Veterinary Parasitology, Faculty of Veterinary Science, Liverpool School of Tropical MedicineUniversity of LiverpoolLiverpool UK
  2. 2.Department of Veterinary Clinical Science, Faculty of Veterinary ScienceUniversity of LiverpoolLeahurstUK
  3. 3.Animal Parasitic Diseases Laboratory, Agricultural Research ServiceUS Department of AgricultureBeltsvilleUSA
  4. 4.Department of Veterinary Clinical Studies, Faculty of Veterinary MedicineUniversity of GlasgowGlasgowUK
  5. 5.Division of ParasitologyMoredun Research InstitutePentlands Science ParkUK

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