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Parasitology Research

, Volume 95, Issue 3, pp 218–223 | Cite as

Schistosoma japonicum migration through mouse skin compared histologically and immunologically with S. mansoni

  • Lin Wang
  • Yong-Long Li
  • Zvi Fishelson
  • John R. Kusel
  • Andreas Ruppel
Original Paper

Abstract

The migration of Schistosoma japonicum and S. mansoni through mouse skin epidermis and dermis was compared by immunofluorescence techniques from 4 to 22 h after infection. At all times, the percentage of parasites detected in the dermis was significantly higher for S. japonicum than for S. mansoni. Thus, S. japonicum migrates more rapidly very early after infection. This agrees with the quicker migration observed previously by this species for later times. Both species expressed antigens related to the cercarial glycocalyx on the parasite body and antigenically detectable elastase in the acetabular glands, at least until 22 h after infection. Bot sets of antigens were also left as “traces” in cercarial migration channels in the skin as well as in skin tissue in the absence of detectable worms or migration channels. The data further substantiate differences between schistosome species in the speed of migration, and suggest that glycocalyx-related antigens and cercarial elastase continue to be expressed for at least 1 day after infection.

Keywords

Mouse Skin Evans Blue Skin Penetration Schistosoma Japonicum Skin Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work received financial support from the German Academic Exchange Service (DAAD), the Ministry of Science and Art Baden-Württemberg, Germany (cooperation Y.L.L. and A.R.), and by a grant from the Federal Ministry of Science and Technology, Germany, and the National Council for Research and Development, Israel (DISNAT program; cooperation Z.F. and A.R.). We thank Heiko Becher (Heidelberg) for statistical advice. The experiments comply with the current laws of Germany.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Lin Wang
    • 1
    • 2
  • Yong-Long Li
    • 2
  • Zvi Fishelson
    • 3
  • John R. Kusel
    • 4
  • Andreas Ruppel
    • 1
  1. 1.Department of Tropical Hygiene and Public HealthUniversity of HeidelbergHeidelbergGermany
  2. 2.Department of Parasitology, Tongji Medical CollegeHuazhong UniversityWuhanPR China
  3. 3.Department of Cell and Developmental BiologyTel Aviv UniversityTel AvivIsrael
  4. 4.Institute of Biological and Life SciencesUniversity of GlasgowGlasgowUK

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