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Parasitology Research

, Volume 95, Issue 3, pp 224–226 | Cite as

The identification of a Clonorchis sinensis gene encoding an antigenic egg protein

  • Mejeong Lee
  • Young-Bae Chung
  • Suk-Keun Lee
  • Byung-Suk Chung
  • Shunyu Li
  • Min-Ho Choi
  • Sung-Tae HongEmail author
Short Communication

Abstract

The cDNA library of Clonorchis sinensis was screened for genes encoding antigenic proteins by using sera from clonorchiasis patients. A gene of 888 bp encoding a 28-kDa protein (Cs28) was cloned and found to contain a high percentage of glycine (20%), tyrosine (11%), and lysine (11%). The amino acid sequence of Cs28 showed 60% homology with the vitelline B precursor protein of Opisthorchis viverrini and of 33% homology with the vitelline B1 and B2 proteins of Fasciola hepatica. A strong positive reaction was observed in the intrauterine eggs of adult C. sinensis by immunohistochemical analysis using specific immune sera against recombinant Cs28 protein (rCs28). By immunoblot analysis, rCs28 displayed an antigenic reaction with 73% of the serum samples from 115 cases of clonorchiasis. In addition, it cross-reacted with the sera of 77.5% of 40 opisthorchiasis cases, 90% of 20 schistosomiasis cases, and 50% of 10 paragonimiasis cases. However, no cross-reactions were observed with the sera of sparganosis or cysticercosis patients. In conclusion, the Cs28 protein was identified as an egg protein of C. sinensis and as an antigen common to the trematode species examined.

Keywords

Schistosomiasis Schistosoma Japonicum Clonorchiasis Trematode Infection Strong Positive Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Mejeong Lee
    • 1
    • 2
  • Young-Bae Chung
    • 1
    • 3
  • Suk-Keun Lee
    • 4
  • Byung-Suk Chung
    • 1
  • Shunyu Li
    • 1
    • 5
  • Min-Ho Choi
    • 1
  • Sung-Tae Hong
    • 1
    Email author
  1. 1.Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine and Institute of Endemic DiseasesSeoul National University Medical Research CenterSeoulKorea
  2. 2.Department of CardiologyNagoya University Graduate School of MedicineNagoyaJapan
  3. 3.Department of Parasitology, College of MedicineCheju National UniversityJejuKorea
  4. 4.Department of Oral Pathology, College of DentistryKangnung National UniversityKangneungKorea
  5. 5.Department of Parasitology, College of MedicineYanbian UniversityYanji P.R. China

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