Advertisement

Parasitology Research

, Volume 95, Issue 2, pp 145–149 | Cite as

A comparison of small-subunit ribosomal RNA gene sequences of bovine Babesia species transmitted by Haemaphysalis spp. in China

  • Jianxun Luo
  • Hong Yin
  • Guiquan Guan
  • Dongying Yang
  • Aihong Liu
  • Miling Ma
  • Zhijie Liu
  • Zhisheng Dang
  • Guangyuan Liu
  • Qi Bai
  • Wenshun Lu
  • Puyan Chen
Original Paper

Abstracts

The ribosomal small-subunit RNA gene sequences of six Chinese Babesia stocks infective to cattle, including a Babesia bigemina isolate, a B. bovis isolate, two B. ovata isolates, a Babesia sp. Wenchuan isolate and a B. major isolate, were compared and analyzed. The target DNA segment was amplified by polymerase chain reaction and the product ligated into the pGEM-T Easy vector for sequencing. The length of the 18S rRNA gene of all Babesia species involved in this study varied between 1,653 and 1,693 bp. The phylogenetic trees were inferred based on the 18S rRNA sequence of the Chinese isolates as well as other species of Babesia available in GenBank. The results showed that the B. ovata transmitted by Haemaphysalis longicornis and Babesia sp. Wenchuan isolate were confined to the same group as B. ovata Korea, with an identity among them of >96.5%, while B. major transmitted by H. punctata was situated in another branch, and identity with other bovine Babesia species was less than 92.5%. B. ovata should, therefore, be a valid species, differing from B. major according to the 18S rRNA gene sequence.

Keywords

Babesia Babesiosis Babesia Species Babesia Parasite ssrRNA Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was partially supported by the National Natural Sciences Foundation of China (no. 39770567), the International Foundation for Sciences (no. AB/11845R) and the ADDAV project (no. ICA4-CT-2000-30028) of the INCO-DEV Programme of the European Commission. We are also indebted to Dr. Theo de Waal, University College Dublin, Faculty of Veterinary Medicine, Dept. of Veterinary Microbiology and Parasitology, Ireland, for his critical correction of this manuscript.

References

  1. Allsopp MT, Cavalier-Smith T, De Waal DT, Allsopp BA (1994) Phylogeny and evolution of the piroplasms. Parasitology 108:147–152Google Scholar
  2. Bai Q, Yin SX, Chen ZH, Liu GY, Zhou JY (1987) Isolation of single species of Babesia bigemina (in Chinese). Chin J Vet Sci Technol 9:25–27Google Scholar
  3. Bai Q, Liu GY, Zhang L, Zhou JY (1990) Discovery and isolation of Babesia ovata in China (in Chinese). Chin J Vet Sci 12:2–4Google Scholar
  4. Bai Q, Liu GY, Zhou JY, Zhang L (1991) Isolation of single species of Babesia bovis (in Chinese). Chin J Vet Sci Technol 1:20–21Google Scholar
  5. Bai Q, Liu GY, Zhang L, Zhou JY (1992) Experiments on the transmission of Babesia ovata (in Chinese). Acta Vet Zootec Sin 23920:166–171Google Scholar
  6. Bai Q, Liu GY, Han GF, Hui Y (1994) Isolation and complementary transmission of Babesia ovata in Zhangjiachuan of Gansu province (in Chinese). Chin J Vet Sci Technol 24:9–10Google Scholar
  7. Bai Q, Liu GY, Yin H, Zhao QZ, Liu DK, Ren JX, Li X (2002) Theileria sinensis nov. sp.: study on molecular taxonomy ( in Chinese). Acta AnimHusband Vet Sci 33: 185–190Google Scholar
  8. Brocklesby DW, Barnett SF (1970) Large Babesia species transmitted to splenectomised calves by field collections of British ticks (Haemaphysalis punctata). Nature 228:1215Google Scholar
  9. Higuchi S, Bai Q, Liu GY, Li DC, Wang XL, Yan ZT (1991) Studies on Babesia sp. isolated from cattle in Henan, China ( in Chinese). Bull Vet Coll P L A 11:63–67Google Scholar
  10. Lu WS, Yin H, Lu WX, Yu F, Zhang QC, Dou HF (1988) Discovery of Babesia major from cattle and confirmation of its transmitting vector tick in China ( in Chinese). Chin J Vet Sci Technol 12:11–14Google Scholar
  11. Lu WS, Yin H, Lu WX, Yu F, Zhang QC, Dou HF (1990) Experimental studies on the transovarial transmission of Babesia major from bovine by tick Haemaphysalis longicornis ( in Chinese). Chin J Vet Sci Technol 6:5–6Google Scholar
  12. Lu WS, Yin H, Luo JX, Lu WX, Zhang QC, Dou HF (1992) Discovery of Babesia major in Xinjiang ( in Chinese). Symposium of the 3rd Meeting of Chinese Society of Veterinary Parasitology, p 220Google Scholar
  13. Minami T, Ishihara T (1980) Babesia ovata sp. n. isolate from cattle in Japan. Natl Inst Anim Health 20:101–113Google Scholar
  14. Morzaria SP, Brocklesby DW, Harradine DL (1977) Experimental transmission of Babesia major by Haemaphysalis punctata. Res Vet Sci 23:261–262Google Scholar
  15. Reddy GR, Chakrabarti D, Yowell CA, Dame JB (1991) Sequence microheterogeneity of the three small subunit ribosomal RNA genes of Babesia bigemina: expression in erythrocyte culture. Nucleic Acids Res 19:3641–3645Google Scholar
  16. Yin H, Lu WS, Luo JX, Zhang QC, Lu WX, Dou HF (1996) Experiments on the transmission of Babesia major and Babesia bigemina by Haemaphysalis punctata. Vet Parasitol 67:89–98CrossRefGoogle Scholar
  17. Yin H, Lu WS, Zhang QC, Lu WX, Luo JX, Liu QY, Du ZM (2000) Experimental transmission of some bovine and ovine tick borne haemoprotozoans in Gansu province (in Chinese). Chin J Vet Parasitol 8:17–19Google Scholar
  18. Yin H, Liu GY, Luo JX, Guan GQ, Ma ML, Ahmed J, Bai Q (2004) Phylogenetic analysis of Theileria species transmitted by Haemaphysalis qinghaiensis. Parasitol Res 92:36–42CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Jianxun Luo
    • 1
    • 2
  • Hong Yin
    • 1
  • Guiquan Guan
    • 1
  • Dongying Yang
    • 1
  • Aihong Liu
    • 1
  • Miling Ma
    • 1
  • Zhijie Liu
    • 1
  • Zhisheng Dang
    • 1
  • Guangyuan Liu
    • 1
  • Qi Bai
    • 1
  • Wenshun Lu
    • 1
  • Puyan Chen
    • 2
  1. 1.Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesGansuPeople’s Republic of China
  2. 2.Institute of Animal Medicine ScienceNanjing Agricultural UniversityJiangsuPeople’s Republic of China

Personalised recommendations