Posttranslational modifications of α-tubulin of Toxoplasma gondii
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The posttranslational modifications of α-tubulin of Toxoplasma gondii were characterized by antibodies and biochemical analysis of the carboxy-terminal peptide. α-Tubulin is acetylated and glutamylated. Side chains with up to three glutamate residues are linked to Glu445 of T. gondii α-tubulin. The data suggest that the site of glutamylation on α-tubulin is conserved over a broad range of species.
KeywordsTrypanosoma Glutamyl Toxoplasma Gondii Trypanosoma Brucei Glutamate Residue
We thank Bernard Eddé (Montpellier, France), Philippe Huitorel (Villefranche-sur-mer, France), and Joel Rosenbaum (New Haven, USA) for antibody gifts and Klaus Weber (Göttingen) for support and discussion. The experiments comply with the laws of Germany. This study was supported by the Deutsche Forschungsgemeinschaft (DFG).