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Parasitology Research

, Volume 94, Issue 5, pp 371–376 | Cite as

Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil. V. Cytokine concentrations in experimentally infected Wistar rats

  • Hermann Feldmeier
  • Lars-Henrik Witt
  • Stefan Schwalfenberg
  • Ronaldo Albuquerque Ribeiro
  • Fernando Queiroz Cunha
  • Gundel Harms
  • Heinz Mehlhorn
  • Oliver Liesenfeld
  • Jörg Heukelbach
Original Paper

Abstract

Tungiasis is caused by the penetration of the female sand flea Tunga penetrans into the skin of its host. This parasitic skin disease is almost invariably associated with an intense inflammation around embedded fleas, the underlying mechanisms being unknown. A study was undertaken to determine whether Wistar rats can be used as an animal model to assess cytokine kinetics during the natural course of the infection. Laboratory-raised Wistar rats were exposed in cages put on the soil in an area with high human attack rates. Rats were examined daily and blood samples were taken before exposure and at 2, 6, 10, 13, 16 and 20 days after flea penetration. TNF-α, IL-1β, IFN-γ, IL-4, IL-10 and CINC (a rat cytokine- induced neutrophil chemoattractant and member of the IL-8 family) were determined by enzyme immunoassay. The results showed an increasing serum concentration of TNF-α and IL-1β 10–13 days after penetration and a rapid increase in IL-4 2 days after fleas became embedded. During the natural course of the infection, the ratio of the serum concentration of TNF-α to that of IL-10 decreased, indicating a relative increase in the secretion of the anti-inflammatory cytokine. The treatment of lesions with silicone oil abrogated the natural disease course and changed the pattern of cytokine secretion. We conclude that the Wistar rat is an appropriate model to study immune responses in tungiasis.

Keywords

Scabies Mite Infestation Myiasis Flea Species Intense Inflammation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This study was partly supported by the World Health Organization (Geneva, Switzerland) and the PROBRAL CAPES/DAAD academic exchange program. The data are part of a medical thesis by L.-H.W. The secretarial assistance of Michi Feldmeier and the technical assistance of Solvy Wolcke are gratefully acknowledged.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Hermann Feldmeier
    • 1
  • Lars-Henrik Witt
    • 1
  • Stefan Schwalfenberg
    • 1
  • Ronaldo Albuquerque Ribeiro
    • 2
  • Fernando Queiroz Cunha
    • 3
  • Gundel Harms
    • 4
  • Heinz Mehlhorn
    • 5
  • Oliver Liesenfeld
    • 1
  • Jörg Heukelbach
    • 6
  1. 1.Department of Medical Microbiology and Immunology of Infection, Institute of Infection MedicineCharité University Medicine BerlinBerlinGermany
  2. 2.Department of Pharmacology and Physiology, School of MedicineFederal University of CearáFortalezaBrazil
  3. 3.Department of PharmacologyFederal University of São PauloRibeirão PretoBrazil
  4. 4.Institute of Tropical MedicineCharité University Medicine BerlinBerlinGermany
  5. 5.Department of Zoology and ParasitologyHeinrich-Heine-UniversityDüsseldorfGermany
  6. 6.Department of Community Health, Medical SchoolFederal University of Ceará and Mandacaru FoundationFortalezaBrazil

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