Parasitology Research

, Volume 94, Issue 1, pp 53–60

The involvement of an integrin-like protein and protein kinase C in amoebic adhesion to fibronectin and amoebic cytotoxicity

  • Kyu-Lee Han
  • Hyun-Ju Lee
  • Myeong Heon Shin
  • Ho-Joon Shin
  • Kyung-Il Im
  • Soon-Jung Park
Original Paper

DOI: 10.1007/s00436-004-1158-9

Cite this article as:
Han, KL., Lee, HJ., Shin, M.H. et al. Parasitol Res (2004) 94: 53. doi:10.1007/s00436-004-1158-9

Abstract

Adherence of a pathogen to the host cell is one of the critical steps in microbial infections. Naegleria fowleri, a causative agent of primary amoebic meningoencephalitis in humans, is expected to interact with extracellular components of the host, such as fibronectin, in a receptor-mediated mode. In this study, we investigated the interaction between N. fowleri and fibronectin to understand its cytopathology. In binding assays using immobilized fibronectin, the number of amoebae bound to fibronectin was increased compared to the controls, and was dependent on the amount of coated fibronectin present. A fibronectin binding protein of 60 kDa was found in extracts of N. fowleri. Western blot and immunolocalization assays using integrin α5/FnR antibodies showed that a 60 kDa protein reacted with the antibodies in extracts of N. fowleri, which was localized on the surface of N. fowleri. Preincubation of N. fowleri with the integrin antibodies significantly inhibited amoebic binding to fibronectin and cytotoxicity to the CHO cells. Additionally, protein kinase C activity was detected in the extract of N. fowleri. When N. fowleri was pretreated with protein kinase C activator or inhibitor, the abilities of amoebic adhesion to fibronectin and cytotoxicity to the host cells were markedly affected compared to untreated amoebae. These results suggest that an amoebic integrin-like receptor and protein kinase C play important roles in amoebic cellular processes in response to fibronectin.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Kyu-Lee Han
    • 1
  • Hyun-Ju Lee
    • 1
  • Myeong Heon Shin
    • 1
  • Ho-Joon Shin
    • 2
  • Kyung-Il Im
    • 1
  • Soon-Jung Park
    • 1
  1. 1.Department of Parasitology and Institute of Tropical Medicine, The Brain Korea 21 ProjectYonsei University College of MedicineSeoulKorea
  2. 2.Department of MicrobiologyAjou University School of MedicineSuwonKorea

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