Parasitology Research

, Volume 93, Issue 2, pp 127–130 | Cite as

Echinococcus granulosus strain typing in Bulgaria: the G1 genotype is predominant in intermediate and definitive wild hosts

  • Isabel Breyer
  • Dimitrina Georgieva
  • Rossitza Kurdova
  • Bruno GottsteinEmail author
Original Paper


Addressing the genetic variability in Echinococcus granulosus is epidemiologically important, as strain characteristics may influence the local transmission patterns of zoonotic cystic echinococcosis. To classify the genotype(s) present in intermediate (pig, cattle and sheep) and definitive (jackal and wolf) hosts in Bulgaria, a DNA-based approach was used to assess parasite protoscoleces or strobiles. Genes corresponding to coding and non-coding regions of the nuclear and mitochondrial genome (ND-1, HBX, Act II, AgB-1) were amplified by PCR and subsequently sequenced. The sequences resolved were all found to be identical to those published for the common sheep strain of E. granulosus, indicating that the G1 genotype is predominant in Bulgaria. One microvariant for ND-1 was found in the pig isolates; however no epidemiological significance was attributed to this finding.


Hydatid Cyst Definitive Host Cystic Echinococcosis Echinococcus Granulosus Cystic Echinococcosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the Swiss National Science Foundation (SCOPES No. 7IP062584 and research grant no. 31-63615.00) and the EU EchinoRisk-project QLK2-CT-2001-01995 (BBW no. 00.0586-1).


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Isabel Breyer
    • 1
  • Dimitrina Georgieva
    • 2
  • Rossitza Kurdova
    • 3
  • Bruno Gottstein
    • 1
    Email author
  1. 1.Institute of ParasitologyUniversity of BerneBerneSwitzerland
  2. 2.Veterinary FacultyThracian UniversityStara ZagoraBulgaria
  3. 3.Department of Parasitology and Tropical MedicineNational Center of Infectious and Parasitic DiseasesSofiaBulgaria

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