Advertisement

Parasitology Research

, Volume 92, Issue 2, pp 159–170 | Cite as

The effect of drugs on cell structure of Tritrichomonas foetus

  • Rodrigo Furtado Madeiro da Costa
  • Marlene BenchimolEmail author
Original Paper

Abstract

The effects of the microtubule affecting drugs taxol, nocodazole and colchicine on the cell cycle and ultrastructure of Tritrichomonas foetus, a protist parasite of cattle, were studied. Alterations in the cytoskeleton, motility and organellar ultrastructure were followed using anti-tubulin antibodies and fluorescence microscopy, scanning- and transmission-electron microscopy. Flow cytometry was also used to analyze the effect of the drugs on the cell cycle. T. foetus was treated with 10 μM taxol, 15 μM nocodazole or 1.5 mM colchicine for 12 h. The first effect observed was pseudocyst formation and alterations in cell motility. The cell cycle was affected and the cells have blocked cytokinesis, but not karyokinesis. The behavior of Golgi, hydrogenosomes and vacuoles was analyzed. The following effects were seen following drug treatments: (1) cell motility was altered and flagella internalized; (2) microtubules of the pelta-axostyle complex were not depolymerized and the axostyle assumed a curved form; (3) hydrogenosomes were of abnormal size and shape; (4) cells became multinucleate; (5) the division process was blocked in cytokinesis; (6) autophagic vacuoles containing a large amount of microtubules were seen; (7) axoneme organization was altered; (8) zoids were formed; (9) signs of cell death, such as membrane blebbing, were observed.

Keywords

Colchicine Taxol Nocodazole Autophagic Vacuole Colchicine Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work has been supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Programa de Núcleos de Exelência (PRONEX) and Associação Universitária Santa Úrsula (AUSU).

References

  1. Batista MCC, Benchimol M, Cunha e Silva LN, De Souza W (1988) Localization of acetylated α-tubulin in Tritrichomonas foetus and Trichomonas vaginalis. Cell Struct Funct 13:445–453PubMedGoogle Scholar
  2. Baum SG, Wittner M, Nadler JP, Horwitz SB, Dennis JE, Schiff PB, Tanowitz HB (1981) Taxol, a microtubule stabilizing agent, blocks the replication of Trypanosoma cruzi. Proc Natl Acad Sci USA 78:4571–4575PubMedGoogle Scholar
  3. Benchimol M (1996) Hydrogenosome autophagy: an ultrastructural and cytochemical study. Biol Cell 91:165–174CrossRefGoogle Scholar
  4. Benchimol M (2001) Hydrogenosome morphological variation induced by fibronectin and other drugs in Tritrichomonas foetus and Tritrichomonas vaginalis. Parasitol Res 87:215–222Google Scholar
  5. Benchimol M, DeSouza W (1987) Structural analysis of the cytoskeleton of Tritrichomonas foetus. J Submicrosc Cytol 19:139–147PubMedGoogle Scholar
  6. Benchimol M, Diniz JAP, Ribeiro K (2000) The fine structure of the axostyle and its associations with organelles in Trichomonads. Tissue Cell 32:178–187CrossRefPubMedGoogle Scholar
  7. Benchimol M, Ribeiro KC, Mariante RM, Alderete JF (2001) Structure and division process of the Golgi complex in Trichomonas vaginalis and Tritrichomonas foetus. Eur J Cell Biol 80:593–607PubMedGoogle Scholar
  8. Boggild AK, Sundermann CA, Estridge BH (2002) Localization of post-translationally modified alpha-tubulin and pseudocyst formation in tritrichomonads. Parasitol Res 88:468–474CrossRefPubMedGoogle Scholar
  9. Brugerolle G (1975) Étude de la cryptopleuromitose et de la morphogènese de division chez plusieurs Genres de trichomonadines primitives. Protistologica 4:457–468Google Scholar
  10. Capuccinelli P, Varesio L (1975) The effect of cytochalasin B, colchicine and vinblastine on the adhesion of Trichomonas vaginalis to glass surfaces. Int J Parasitol 5:57–61CrossRefPubMedGoogle Scholar
  11. Cole NB, Sciaky N, Marotta A, Song J, Lippincott-Schwartz (1996) Golgi dispersal during microtubule disruption: regeneration of Golgi stacks at peripheral endoplasmic reticulum exit site. Mol Biol Cell 7:631–650PubMedGoogle Scholar
  12. Delgado-Viscogliosi P, Brugerolle G, Viscogliosi E (1996) Tubulin post-translational modifications in the primitive protist Trichomonas vaginalis. Cell Motil Cytoskeleton 33:288–297CrossRefPubMedGoogle Scholar
  13. Diamond LS (1957) The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 43:488–490Google Scholar
  14. Granger BL, Warwood SJ, Benchimol M, De Souza W (2000) Transient invagination of flagella by Tritrichomonas foetus. Parasitol Res 86:699–709PubMedGoogle Scholar
  15. Honigberg MB, Brugerolle G (1990) Structure. In: Honigberg MB (ed) Trichomonads parasitic in humans. Springer, New York Berlin Heidelberg, pp 5–35Google Scholar
  16. Honigberg MB, Mattern FTC, Daniel AW (1971) Fine structure of the mastigont system in Tritrichomonas foetus. J Protozool 18:183–198PubMedGoogle Scholar
  17. Juliano C, Monaco G, Rubino S, Cappuccinelli P (1986a) Inhibition of Tritrichomonas vaginalis replication by the microtubule stabilizer taxol. J Protozool 33:255–260PubMedGoogle Scholar
  18. Juliano C, Rubino S, Zicconi D, Cappuccinelli P (1986b) An immunofluorescence study of the microtubule organization in Trichomonas vaginalis using antitubulin antibodies. J Protozool 33:56–59PubMedGoogle Scholar
  19. Kreis TE, Goodson HV, Perez F, Rönnolm R (1997) Golgi apparatus-cytoskeleton interactions. In: Berger EG, Roth J (eds) The Golgi apparatus. Birkhäuser, Basel, pp 179–193Google Scholar
  20. Kulda J (1999) Trichomonads, hydrogenosomes and drug resistance. Int J Parasitol 29:199–212CrossRefPubMedGoogle Scholar
  21. Lawrence PB, Brown JW (1992) Autophagic vacuoles rapidly fuse with pre-existing lysosomes in cultured hepatocytes. J Cell Sci 102:515–526PubMedGoogle Scholar
  22. Lipman NS, Lampen N, Nguyen HT (1999) Identification of pseudocysts of Tritrichomonas muris in Armenian hamsters and their transmission to mice. Lab Anim Sci 49:313–315PubMedGoogle Scholar
  23. Lopes LC, Ribeiro KC, Benchimol M (2001) Immunolocalization of tubulin isoforms and post-translational modifications in the protists Tritrichomonas foetus and Trichomonas vaginalis. Histochem Cell Biol 116:17–29PubMedGoogle Scholar
  24. Mariante RM, Guimarães CA, Linden R, Benchimol M (2003) Hydrogen peroxide induces caspase activation and programmed cell death in the amitochondrial Tritrichomonas foetus. Histochem Cell Biol 120:129–141CrossRefPubMedGoogle Scholar
  25. Marzella L, Glausmann H (1980) Increased degradation in rat liver induced by vinblastine II. Morphologic characterization. Lab Invest 42:18–27PubMedGoogle Scholar
  26. Mattern CFT, Daniel WA (1980) Tritrichomonas muris in the hamster: pseudocysts and the infection of newborn. J Protozool 27:435–439PubMedGoogle Scholar
  27. Morrissette NS, Sibley LD (2002) Disruption of microtubules uncouples budding and nuclear division in Toxoplasma gondii. J Cell Sci 115:1017–1025PubMedGoogle Scholar
  28. Müller M (1988) Energy metabolism of protozoa without mitochondria. Annu Rev Microbiol 42:465–488CrossRefPubMedGoogle Scholar
  29. Müller M (1993) The hydrogenosome. J Gen Microbiol 139:2879–2889PubMedGoogle Scholar
  30. Noël C, Gerbod D, Delgado-Viscogliosi P, Fast NM, Younes AB, Chose O, Roseto A, Capron M, Viscogliosi E (2003) Morphogenesis during division and griseoulvin-induced changes of the microtubular cytoskeleton in the parasitic protist, Trichomonas vaginalis. Parasitol Res 89:487–494PubMedGoogle Scholar
  31. Pereira-Neves A, Ribeiro KC, Benchimol M (2003) Pseudocysts in Trichomonads—new insights. Protist 154:313–329CrossRefGoogle Scholar
  32. Ploubidou A, Robinson DR, Docherty RC, Ogbadoyi EO, Gull K (1999) Evidence for novel cell cycle checkpoints in trypanosomes: kinetoplast segregation and cytokinesis in the absence of mitosis. J Cell Sci 112:4641–4650PubMedGoogle Scholar
  33. Ribeiro KC, Monteiro-Leal LH, Benchimol M (2000) Contributions of the axostyle and flagella to the closed mitosis of Tritrichomonas foetus and Trichomonas vaginalis. J Eukaryot Microbiol 47:481–492PubMedGoogle Scholar
  34. Ribeiro KC, Arnholdt ACV, Benchimol M (2002) Tritrichomonas foetus: induced division synchrony by hydroxyurea. Parasitol Res 88:627–631CrossRefPubMedGoogle Scholar
  35. Riley DE, Krieger JN, Miner D, Rabinovitch PS (1994) Trichomonas vaginalis: dominant G2 period and G2 phase arrest in a representative of an early branching eukaryotic lineage. J Eukaryot Microbiol 41:408–414PubMedGoogle Scholar
  36. Samuels R (1957) Studies of Tritrichomonas batachorum (perty) 2. Normal mitosis and morphogenesis. Trans Am Microsc Soc 76:295–307Google Scholar
  37. Samuels R (1959) Studies of Tritrichomonas batachorum 3. Abnormal mitosis and morphogenesis. Trans Am Microsc Soc 78:49–65Google Scholar
  38. Schliwa M, Blerkom J (1981) Structural interaction of cytoskeletal components. J Cell Biol 90:222–235PubMedGoogle Scholar
  39. Silva-Filho FC, De Souza W (1986) Effect of colchicine, vinblastine and cytochalasin B on cell surface anionic sites of Tritrichomonas foetus. J Protozool 33:6–10PubMedGoogle Scholar
  40. Thyberg J, Moskalewski S (1999) Roles of microtubules in the organization of the Golgi complex. Exp Cell Res 246:263–279PubMedGoogle Scholar
  41. Viscogliosi E, Brugerolle G (1994) Cytoskeleton in trichomonads. III Study of the morphogenesis during division by using monoclonal antibodies against cytoskeletal structures. Eur J Protistol 30:129–138Google Scholar
  42. Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT (1971) Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc 93:2325–2327PubMedGoogle Scholar
  43. Wenrich DH (1939) The morphology of Trichomonas vaginalis. Vol Jub S Yoshida Osaka 2:65–76Google Scholar
  44. Yang W, Storrie B (1998) Scattered Golgi elements during microtubule disruption are initially enriched in trans Golgi proteins. Mol Biol Cell 9:191–207PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Rodrigo Furtado Madeiro da Costa
    • 1
  • Marlene Benchimol
    • 2
    Email author
  1. 1.Programa de Pós Graduação em Ciências MorfológicasUniversidade Federal Do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Laboratório de Ultraestrutura CelularUniversidade Santa ÚrsulaRio de JaneiroBrazil

Personalised recommendations