Parasitology Research

, Volume 91, Issue 5, pp 378–383

Ribosomal RNA analysis of Babesia odocoilei isolates from farmed reindeer (Rangifer tarandus tarandus) and elk (Cervus elaphus canadensis) in Wisconsin

  • Patricia J. Holman
  • Kylie G. Bendele
  • Lorien Schoelkopf
  • Richard L. Jones-Witthuhn
  • Scott O. Jones
Original Paper

DOI: 10.1007/s00436-003-0984-5

Cite this article as:
Holman, P.J., Bendele, K.G., Schoelkopf, L. et al. Parasitol Res (2003) 91: 378. doi:10.1007/s00436-003-0984-5

Abstract

Piroplasms isolated from a farmed reindeer and elk in Wisconsin were determined to be Babesia odocoilei, based on morphology and ribosomal RNA (rRNA) analysis. Different clinical manifestations were observed in the two host species. The reindeer was parasitemic and exhibited acute babesiosis resulting in death, while the elk showed no parasites in blood smears and no overt clinical signs of babesiosis. B. odocoilei was, however, readily cultured from elk erythrocytes. Small subunit rRNA gene sequences from the two isolates were identical to that previously reported for B. odocoilei. Internal transcribed spacers 1 and 2 and 5.8S rRNA sequence analysis showed an overall identity range of 94.3–98.1% to corresponding sequences from three previously reported B. odocoilei isolates, but the Wisconsin reindeer B. odocoilei shared only 87.3% identity with a previously reported Babesia sp. isolated from a reindeer in California (RD61).

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Patricia J. Holman
    • 1
  • Kylie G. Bendele
    • 1
  • Lorien Schoelkopf
    • 1
  • Richard L. Jones-Witthuhn
    • 2
  • Scott O. Jones
    • 3
  1. 1.Department of Veterinary Pathobiology, Texas Veterinary Medical CenterTexas A&M UniversityCollege StationUSA
  2. 2.Kinni-Croix Veterinary ClinicRiver FallsUSA
  3. 3.Wisconsin Animal Health LaboratoryState of Wisconsin Department of Agriculture, Trade and Consumer ProtectionBarronUSA

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