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Parasitology Research

, Volume 90, Issue 4, pp 325–329 | Cite as

Analysis of the Plasmodium vivax apical membrane antigen-1 gene from re-emerging Korean isolates

  • Joon-Yong Chung
  • Eui-Hyun Chun
  • Jin-Ho Chun
  • Weon-Gyu KhoEmail author
Original Paper

Abstract.

Apical membrane antigen-1 is a candidate vaccine component for malaria. In the present study, we investigated the polymorphism of the Plasmodium vivax apical membrane antigen-1 gene (PvAMA-1) in 30 Korean isolates. The polymorphic region of the PvAMA-1 gene, corresponding to nucleotides 324–735 (aa 108–245), was amplified using polymerase chain reaction followed by cloning and sequencing. Two genotypes (SKA and SKG) were identified on the basis of amino acid substitution. These were identical to those of the Chinese isolates. The Korean isolates showed sequence variation at six positions on the basis of the sequence of the Sal1 strain. Of these, variation at position 189 (Glu/Lys) was found only in SKA. These two genotypes were related to the genotype of the circumsporozoite and Duffy binding protein of the Korean isolate. These findings suggest that two genotypes of P. vivax coexist in the endemic area and that the re-emerging parasite in Korea may be related to or have originated in East Asia.

Keywords

Malaria Vivax Malaria Malaria Vaccine Chinese Isolate Korean Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements.

This work was supported by the 2000 Inje University research grant. We acknowledge the technical expertise provided by Eun-Jeong Sim and Seung-Young Hwang. The authors would like to thank Hyewon Lee for editing the manuscript.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Joon-Yong Chung
    • 1
  • Eui-Hyun Chun
    • 1
  • Jin-Ho Chun
    • 2
  • Weon-Gyu Kho
    • 1
    Email author
  1. 1.Department of Parasitology and Institute of Malariology, Inje University College of Medicine, Pusan 614-735, Republic of Korea
  2. 2.Department of Preventive Medicine, Inje University College of Medicine, Pusan 614-735, Republic of Korea

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