Codon optimization improves heterologous expression of a Schistosoma mansoni cDNA in HEK293 cells
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- Hamdan, F.F., Mousa, A. & Ribeiro, P. Parasitol Res (2002) 88: 583. doi:10.1007/s00436-001-0585-0
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Differences in codon usage can seriously hamper the expression of cloned cDNAs in heterologous systems. In this study, we show that the expression of a cloned Schistosoma mansoni cDNA in cultured HEK293 cells was dramatically increased by rewriting a portion of the cDNA according to human preferred codon usage, suggesting that codon optimization is a valuable strategy for improving the heterologous expression of helminth sequences. We further describe a simple modification of a recursive PCR-based method, which allows the rewriting of long stretches of DNA sequence in a single PCR reaction. This method can be used to optimize the codon usage of virtually any DNA from helminths and other parasites.