Parasitology Research

, Volume 88, Issue 6, pp 583–586

Codon optimization improves heterologous expression of a Schistosoma mansoni cDNA in HEK293 cells

  • Fadi F. Hamdan
  • Aisha Mousa
  • Paula Ribeiro
Short Communication

DOI: 10.1007/s00436-001-0585-0

Cite this article as:
Hamdan, F.F., Mousa, A. & Ribeiro, P. Parasitol Res (2002) 88: 583. doi:10.1007/s00436-001-0585-0

Abstract.

Differences in codon usage can seriously hamper the expression of cloned cDNAs in heterologous systems. In this study, we show that the expression of a cloned Schistosoma mansoni cDNA in cultured HEK293 cells was dramatically increased by rewriting a portion of the cDNA according to human preferred codon usage, suggesting that codon optimization is a valuable strategy for improving the heterologous expression of helminth sequences. We further describe a simple modification of a recursive PCR-based method, which allows the rewriting of long stretches of DNA sequence in a single PCR reaction. This method can be used to optimize the codon usage of virtually any DNA from helminths and other parasites.

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  • Fadi F. Hamdan
    • 1
  • Aisha Mousa
    • 1
  • Paula Ribeiro
    • 1
  1. 1.Institute of Parasitology, McGill University, 21, 111 Lakeshore Road, Ste. Anne de Bellevue, Quebec H9X 3V9Canada

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