, 125:87 | Cite as

Investigation of the budding process in Tethya citrina and Tethya aurantium (Porifera, Demospongiae)

  • Elda Gaino
  • Lidia Scalera Liaci
  • Margherita Sciscioli
  • Giuseppe CorrieroEmail author
Original Article


The budding process has been studied in two congeneric Mediterranean species belonging to Tethya from different sampling sites: Marsala and Venice Lagoons (Tethya citrina); Marsala Lagoon and Porto Cesareo Basin (Tethya aurantium). Buds, connected to the adult by a spiculated stalk, differ between the two species in morphology and size, since those of T. citrina are small with elongated bodies, showing only a few spicules protruding from the apical region, whereas those of T. aurantium are round, larger, and show spicules radiating from the peripheral border. In T. citrina, cells with inclusions, varying in electron density and size, represent the main cell types of the buds. In T. aurantium, the cell component shows a major diversification, resulting from spherulous cells, grey cells, vacuolar cells and peculiar micro-vesicle cells. Neither canals nor choanocyte chambers were observed in the buds of the two species. In T. citrina, bud production is similar in both sampling sites. In T. aurantium, budding occurs more rarely in Porto Cesareo Basin, probably in relation with environmental factors, such as the covering of the cortex by sediment and micro-algae. Finally, in the buds of both species, the spicule size does not differ from that of the cortex of the adult sponges, further supporting the main involvement of the cortex in organizing the skeletal architecture of the buds.


Porifera Tethya Bud differentiation Spicules Ultrastructure 


  1. Bavestrello G, Corriero G, Sarà M (1992) Differences between two sympatric species of Tethya (Porifera, Demospongiae) concerning the growth and final form of their megasters. Zool J Linnean Soc 104:81–87CrossRefGoogle Scholar
  2. Bavestrello G, Calcinai B, Ceccati L, Cerrano C, Sarà M (2000) Skeletal development in two species of Tethya (Porifera, Demospongiae). Italian J Zool 67:241–244CrossRefGoogle Scholar
  3. Bergquist PR, Kelly-Borges M (1991) An evaluation of the genus Tethya (Porifera: Demospongiae: Hadromerida) with description of new species from the Southwest Pacific. Beagle 8:37–72Google Scholar
  4. Boury-Esnault N (1970) Un phénomène de bourgeonnement externe chez l’éponge Axinella damicornis (Exper.). Cah Biol Mar 11:491–496Google Scholar
  5. Chen YH, Chen CP, Chang KH (1997) Budding cycle and bud morphology of the globe-shaped sponge Cinachyra australiensis. Zool Studies 36(3):194–200Google Scholar
  6. Connes R (1967) Structure et développement des bourgeons chez l’éponge siliceuse Tethya lyncurium Lamarck. Archs Zool Exp Gén 108:157–195Google Scholar
  7. Connes R (1968) Etude histologique, cytologique et expérimentale de la régénération et de la reproduction asexuée chez Tethya lyncurium Lamarck (= Tethya aurantium (Pallas) (Demosponges). Thèse. Université Montpellier, FranceGoogle Scholar
  8. Connes R (1977) Contribution a l’étude de la gemmulogenèse chez la démosponge marine Suberites domuncula (Olivi) Nardo. Archs Zool Exp Gén 118:391–407Google Scholar
  9. Corriero G (1990) Distribuzione ed ecologia dei Poriferi in ambienti “confinati” mediterranei. Tesi di Dottorato di Ricerca. Università degli Studi di Genova, ItaliaGoogle Scholar
  10. Corriero G, Balduzzi A, Sarà M (1989) Ecological differences in the distribution of two Tethya (Porifera, Demospongiae) species coexisting in a Mediterranean coastal lagoon. Pubbl Staz Zool Napoli (I Mar Ecol) 10:303–315Google Scholar
  11. Corriero G, Sarà M, Vaccaro P (1996) Sexual and asexual reproduction in two species of Tethya (Porifera: Demospongiae) from a Mediterranean coastal lagoon. Mar Biol 126:175–181CrossRefGoogle Scholar
  12. Corriero G, Scalera Liaci L, Nonnis Marzano C, Gaino E (1998) Reproductive strategies of Mycale contarenii (Porifera: Demospongiae). Mar Biol 131:319–327CrossRefGoogle Scholar
  13. Corriero G, Mercurio M, Scalera Liaci L, Marchini A, Occhipinti A (2003) Taxocenosi bentoniche di substrato duro a Venezia: aspetti distribuzionali e modificazioni temporali nel settore settentrionale. Riassunti 64° Congresso Nazionale Unione Zoologica Italiana: 79–80Google Scholar
  14. De Vos C (1965) Le bourgeonnement externe de l’éponge Mycale contarenii. Bull Mus Nat Hist Nat Paris 37:548–555Google Scholar
  15. Ereskovskii AV (2003) Problems of coloniality, modulatity, and individuality in sponges and special features of their morphogeneses during growth and asexual reproduction. Russ J Mar Biol 29(1):46–56CrossRefGoogle Scholar
  16. Fell PE (1993) A. Porifera. In: Adiyodi KG, Adiyodi RG (eds) Asexual propagation and reproductive strategies. Oxford and IBH, New Delhi, pp 1–44Google Scholar
  17. Fromont J (1999) Reproduction of some demosponges in a temperate Australian shallow water habitat. Mem Queensl Mus 44:185–192Google Scholar
  18. Gaino E, Sciscioli M, Lepore E, Scalera Liaci L, Corriero G (2002) Budding in the two Mediterranean Tethya species: morphological and ecological aspects. Boll Mus Ist Biol Univ Genova 66–67:74Google Scholar
  19. Maldonado M, Uriz MJ (1999) Sexual propagation by sponge fragments. Nature 398:476CrossRefGoogle Scholar
  20. Mercurio M (2000) Distribuzione, ecologia e sperimentazione di tecniche di restocking della fauna a Poriferi presso l’insenatura della Strea di Porto Cesareo. Tesi di Dottorato di Ricerca. Università degli Studi di Bari, ItaliaGoogle Scholar
  21. Merejkowski C (1880) Reproduction des Eponges par bourgeonnement extérieur. Arch Zool Exp Gén 8:417Google Scholar
  22. Passeri L (1974) Sedimentazione carbonatica attuale e diagenesi precoce nella laguna di Porto Cesareo (Penisola Salentina). Boll Soc Geol Ital 92(suppl):3–40Google Scholar
  23. Saller U (1990) Formation and construction of asexual buds of the fresh-water sponge Radiospongilla cerebellata (Porifera, Spongillidae). Zoomorphology 109:295–301CrossRefGoogle Scholar
  24. Sarà M (1987) A study on the genus Tethya (Porifera, Demospongiae) and new perspectives in sponge systematics. In: Vacelet J, Boury-Esnault N (eds) Taxonomy of porifera. Springer, Berlin Heidelberg New York, pp 205–225Google Scholar
  25. Sarà M (1988) Two new species of Tethya (Porifera, Demospongiae) from New Caledonia. Bull Mus Hist Nat Paris 4(10):651–659Google Scholar
  26. Sarà M, Mensi P, Manconi R, Bavestrello G, Balletto E (1989) Genetic variability in Mediterranean populations of Tethya (Porifera, Demospongiae). In: Ryland JS, Tyler PA (eds) Reproduction, genetics and distributions of marine organisms. Olsen & Olsen, Fredensborg, pp 293–298Google Scholar
  27. Sarà M, Corriero G, Bavestrello G (1993) Tethya (Porifera, Demospongiae) species coexisting in a Maldivian coral lagoon: taxonomic, genetic and ecological data. Pubbl Staz Zool Napoli (I Mar Ecol) 14(4):341–355Google Scholar
  28. Sconfietti R (1989) Ecological zonation and dynamics of hard-bottom peracarid communities along the lagoon estuary of the river Dese (Lagoon of Venice, Northern Adriatic Sea). Riv Idrobiol 28(1–2):3–31Google Scholar
  29. Simpson TL (1984) The cell biology of sponges. Springer, Berlin Heidelberg New YorkGoogle Scholar
  30. Tsurumi M, Reiswig HM (1997) Sexual versus asexual reproduction in an oviparous rope-form sponge, Aplysina cauliformis (Porifera, Verongidae). Invert Rep Develop 32(1):1–9Google Scholar
  31. Vacelet J (1999) Planktonic armoured propagules of the excavating sponge Alectona (Porifera: Demospongiae) are larvae: evidence from Alectona wallichii and Alectona mesatlantica. Mem Queensl Mus 44:627–642Google Scholar
  32. Weissenfels N (1989) Biologie und Mikroskopische Anatomie der Süsswasserschwamme (Spongillidae). Fisher Press, New YorkGoogle Scholar
  33. Wulff JL (1991) Asexual fragmentation, genotype success, and population dynamics of erect branching sponges. J exp Mar Biol Ecol 149:227–247CrossRefGoogle Scholar
  34. Wulff JL (1995) Effects of a hurricane on survival and orientation of large erect coral reef sponges. Coral Reefs 14:55–61CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Elda Gaino
    • 2
  • Lidia Scalera Liaci
    • 1
  • Margherita Sciscioli
    • 1
  • Giuseppe Corriero
    • 1
    Email author
  1. 1.Dipartimento di Zoologia dell’UniversitàBariItaly
  2. 2.Dipartimento di Biolgia Cellulare ed AmbientalePerugiaItaly

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