Research in Experimental Medicine

, Volume 198, Issue 6, pp 341–347

Superoxide dismutase activity and the effect of N-methly-D-aspartate antagonists on lipid peroxidation in the early phase of cold injury

  • Talat Kırış
  • Aşkin Görgülü
  • Faruk Ünal
  • Ümit Türkoğlu
  • Sabahattin Çobanoğlu
  • Galip Ekuklu
Article

DOI: 10.1007/s004330050116

Cite this article as:
Kırış, T., Görgülü, A., Ünal, F. et al. Res. Exp. Med. (1998) 198: 341. doi:10.1007/s004330050116

Abstract

Free radicals, lipid peroxidation and excitatory amino acids have been implicated in the secondary mechanisms of traumatic brain injury. We used the cold injury model in rats to assess the endogenous activity of the protective enzyme superoxide dismutase (SOD) and the lipid peroxidation level in the contused tissue at an early phase of injury. Furthermore, we treated the rats with two different N-methyl-D-aspartate receptor antagonists, namely MK-801 and CPP, and evaluated their effect on lipid peroxidation in the contused tissue. Rats were divided into four groups: sham, control, treatment 1 and treatment 2 groups (n =16 for each group). Thirty and 60 min after craniectomy or injury, tissue samples were removed. SOD activity didn’t change in this period. However, lipid peroxidation in terms of malondialdehyde (MDA) amount showed a significant increase at 60 min. Fifteen minutes after injury, MK-801 (1 mg/kg), CPP (10 mg/kg) or saline (1 ml) were applied intraperitoneally in treatment 1, treatment 2 and the control groups. Treatment with MK-801 attenuated MDA levels, whereas treatment with CPP did not. The protective effect of MK-801 achieved statistical significance. These results demonstrate that SOD activity does not change in the early period of cold injury. Moreover, these results show that lipid peroxidation increases after 60 min of cold injury, and treatment with MK-801 15 min after injury can prevent this elevation.

Key words

Cold injury Lipid peroxidation MK-801 NMDA antagonist Superoxide dismutase 

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • Talat Kırış
    • 1
  • Aşkin Görgülü
    • 2
  • Faruk Ünal
    • 1
  • Ümit Türkoğlu
    • 3
  • Sabahattin Çobanoğlu
    • 2
  • Galip Ekuklu
    • 4
  1. 1.Department of Neurosurgeryİstanbul University, School of MedicineÇapa İstanbulTurkey
  2. 2.Department of NeurosurgeryTrakya University, School of MedicineEdirneTurkey
  3. 3.Department of Biochemistryİstanbul University, School of MedicineÇapa İstanbulTurkey
  4. 4.Department of Public HealthTrakya University, School of MedicineEdirneTurkey
  5. 5.İstanbulTurkey

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