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Human umbilical cord mesenchymal stem cells-derived exosomal microRNA-181a retards nasopharyngeal carcinoma development by mediating KDM5C

  • Original Article – Cancer Research
  • Published:
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Abstract

Objective

It has been studied that mesenchymal stem cells (MSCs)-derived exosomes could suppress tumor growth in nasopharyngeal carcinoma (NPC) and microRNA-181a (miR-181a) could mediate drug resistance in NPC. Focused on this work, the mechanism of human umbilical cord MSCs (hUC-MSCs)-derived exosomal miR-181a was explored in NPC cell progression.

Methods

NPC tissues and normal tissues were obtained from patients, and miR-181a and KDM5C expression was examined. hUC-MSCs-derived exosomes were extracted, identified and co-cultured with NPC cells (C666-1 and SUNE1). C666-1 cell progression in vitro and/or tumor growth in vivo were examined after incubation with exosomes, miR-181a or lysine-specific demethylase 5C (KDM5C). miR-181a and KDM5C expression were examined in NPC.

Results

miR-181a expression was reduced while KDM5C expression was elevated in NPC. hUC-MSCs-derived exosomes restrained NPC cell growth in vivo and in vitro. Depleting or restoring exosomal miR-181a promoted or delayed NPC cell progression. KDM5C silencing suppressed NPC cell progression.

Conclusion

This study concluded that hUC-MSCs-derived exosomal miR-181a retards NPC development via negatively modulating KDM5C, serving as a candidate reference for the therapy of NPC.

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Acknowledgements

We would like to acknowledge the reviewers for their helpful comments on this paper.

Funding

The financial reports of The Self-funded Research Projects of National Health and Family Planning Commission of Guangxi (NO. Z20170226); Basic Ability Improvement Project for young and middle-aged Teachers in Universities of Guangxi (NO. 2018KY0451); 2018 Guangxi scholarship Fund of Guangxi Education Department; Special Funding for Guangxi Special Experts (No. GRCT[2019]13#); Guangxi Medical High-level Leading Talents Training “139” Project (No. GWKJ[2018] 22#).

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Authors and Affiliations

  1. School of the Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410128, China

    Jin Liu

  2. Department of Otolaryngology Head and Neck Surgery, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China

    Jin Liu

  3. Department of Pathophysiology, Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China

    Mingyi Zhu

  4. Hunan University of Chinese Medicine, No.113 Shaoshan Middle Road, Changsha, 410208, Hunan, China

    Qianli Tang

  5. Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China

    Qianli Tang

Authors
  1. Jin Liu
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  2. Mingyi Zhu
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  3. Qianli Tang
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Correspondence to Qianli Tang.

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Supplementary Information

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432_2021_3684_MOESM1_ESM.eps

Supplementary file1 (EPS 14520 KB) Supplementary Figure 1 hUC-MSCs-derived exosomes restrict SUNE1 cell growth in vitro. A. CCK-8 assay detected SUNE1 cell proliferation; B. Colony formation assay detected SUNE1 cell colony formation ability; C. Flow cytometry detected SUNE1 cell cycle; D. Flow cytometry detected SUNE1 cell apoptosis E. Transwell assay detected SUNE1 cell migration; F. Transwell assay detected SUNE1 cell invasion; the data in the figure were all measurement data, showing as mean ± standard deviation (n = 3). * P < 0.05 compared with the PBS group. The comparison between two groups was analyzed by independent sample t test.

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Liu, J., Zhu, M. & Tang, Q. Human umbilical cord mesenchymal stem cells-derived exosomal microRNA-181a retards nasopharyngeal carcinoma development by mediating KDM5C. J Cancer Res Clin Oncol 147, 2867–2877 (2021). https://doi.org/10.1007/s00432-021-03684-6

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  • DOI: https://doi.org/10.1007/s00432-021-03684-6

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