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Journal of Cancer Research and Clinical Oncology

, Volume 142, Issue 6, pp 1261–1271 | Cite as

Effects of paclitaxel on permanent head and neck squamous cell carcinoma cell lines and identification of anti-apoptotic caspase 9b

  • Regina MaushagenEmail author
  • Stefan Reers
  • Ann-Christin Pfannerstill
  • Angelina Hahlbrock
  • Roland Stauber
  • Ramtin Rahmanzadeh
  • Dirk Rades
  • Ralph Pries
  • Barbara Wollenberg
Original Article – Cancer Research

Abstract

Purpose

Paclitaxel is an effective chemotherapeutic agent against various human tumors inducing apoptosis via binding to β-tubulin of microtubules and arresting cells mainly in the G2/M phase of the cell cycle. However, the underlying specific molecular mechanisms of paclitaxel on head and neck squamous cell carcinoma (HNSCC) have not been identified yet.

Methods

The apoptotic effects and mechanisms of paclitaxel on different permanent HPV-negative HNSCC cell lines (UT-SCC-24A, UT-SCC-24B, UT-SCC-60A and UT-SCC-60B) were determined by flow cytometry assays, polymerase chain reaction analysis, immunofluorescence-based assays and sequencing studies.

Results

Paclitaxel induced a G2/M arrest in HNSCC cell lines followed by an increased amount of apoptotic cells. Moreover, the activation of caspase 8, caspase 10 and caspase 3, and the loss of the mitochondrial outer membrane potential could be observed, whereas an activation of caspase 9 could barely be detected. The efficient activation of caspase 9 was not affected by altered methylation patterns. Our results can show that the promoter region of apoptotic protease activating factor 1 (Apaf-1) was not methylated in the HNSCC cell lines. By sequencing analysis two isoforms of caspase 9, the pro-apoptotic caspase 9 and the anti-apoptotic caspase 9b were identified. The anti-apoptotic caspase 9b is missing the catalytic site and acts as an endogenous inhibitor of apoptosis by blocking the binding of caspase 9 to Apaf-1 to form the apoptosome.

Conclusion

Our data indicate the presence of anti-apoptotic caspase 9b in HNSCC, which may serve as a promising target to increase chemotherapeutic apoptosis induction.

Keywords

Apoptosis Head and neck cancer Paclitaxel Caspases Caspase 9b 

Notes

Acknowledgments

This work was supported by Grants from the Werner and Klara Kreitz-Stiftung.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary material 1 (TIFF 2046 kb)
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Supplementary material 2 (TIFF 6262 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Regina Maushagen
    • 1
    Email author
  • Stefan Reers
    • 2
  • Ann-Christin Pfannerstill
    • 3
  • Angelina Hahlbrock
    • 4
  • Roland Stauber
    • 4
  • Ramtin Rahmanzadeh
    • 5
  • Dirk Rades
    • 6
  • Ralph Pries
    • 1
  • Barbara Wollenberg
    • 1
  1. 1.Department of Otorhinolaryngology, Clinic for ENT and HNSUniversity Hospital of Schleswig-HolsteinLübeckGermany
  2. 2.Department of Cardiology and AngiologyUniversity Hospital MünsterMünsterGermany
  3. 3.Department for Nephrology and HypertensionUniversity Hospital of Schleswig-HolsteinKielGermany
  4. 4.Department of Molecular OncologyUniversity Medical Center MainzMainzGermany
  5. 5.Institute of Biomedical OpticsUniversity of LübeckLübeckGermany
  6. 6.Department of Radiation OncologyUniversity Hospital of Schleswig-HolsteinLübeckGermany

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