MiR-345 suppresses proliferation, migration and invasion by targeting Smad1 in human prostate cancer
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The roles of dysregulated microRNAs in prostate cancer metastasis are still unknown. In this study, we found that the expression of miR-345 was significantly downregulated in prostate cancer and clinical prostate cancer tissues.
Materials, methods and results
Overexpression of miR-345 in prostate cancer cells suppressed proliferation, migration and invasion. Using nude mice model, we revealed that miR-345 inhibits the growth of prostate cancer cells in vivo and in vitro. Furthermore, we identified and validated Smad1 as a direct target of miR-345. Ectopic expression of Smad1 without its 3′-UTR rescued miR-345-induced cell migration and invasion inhibition.
Taken together, our data suggest that miR-345 exerts a suppressive effect on prostate cancer proliferation, invasion and migration through downregulation of Smad1.
KeywordsProstate cancer microRNA miR-345 Smad1
Compliance with ethical standards
Conflict of interest
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