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Journal of Cancer Research and Clinical Oncology

, Volume 142, Issue 1, pp 213–224 | Cite as

MiR-345 suppresses proliferation, migration and invasion by targeting Smad1 in human prostate cancer

  • Qi-guang Chen
  • Wei Zhou
  • Tao Han
  • Shu-qi Du
  • Zhen-hua Li
  • Zhe Zhang
  • Guang-yi Shan
  • Chui-ze KongEmail author
Original Article – Cancer Research

Abstract

Introduction

The roles of dysregulated microRNAs in prostate cancer metastasis are still unknown. In this study, we found that the expression of miR-345 was significantly downregulated in prostate cancer and clinical prostate cancer tissues.

Materials, methods and results

Overexpression of miR-345 in prostate cancer cells suppressed proliferation, migration and invasion. Using nude mice model, we revealed that miR-345 inhibits the growth of prostate cancer cells in vivo and in vitro. Furthermore, we identified and validated Smad1 as a direct target of miR-345. Ectopic expression of Smad1 without its 3′-UTR rescued miR-345-induced cell migration and invasion inhibition.

Conclusion

Taken together, our data suggest that miR-345 exerts a suppressive effect on prostate cancer proliferation, invasion and migration through downregulation of Smad1.

Keywords

Prostate cancer microRNA miR-345 Smad1 

Notes

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Qi-guang Chen
    • 1
  • Wei Zhou
    • 2
  • Tao Han
    • 3
  • Shu-qi Du
    • 1
  • Zhen-hua Li
    • 1
  • Zhe Zhang
    • 1
  • Guang-yi Shan
    • 4
  • Chui-ze Kong
    • 1
    Email author
  1. 1.Department of UrologyThe First Affiliated Hospital of China Medical UniversityShenyangChina
  2. 2.Department of Diagnostic RadiologyGeneral Hospital of Shenyang Military RegionShenyangChina
  3. 3.Department of OncologyGeneral Hospital of Shenyang Military RegionShenyangChina
  4. 4.Department of UrologyLiaoNing Cancer Hospital and InstituteShenyangChina

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