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Journal of Cancer Research and Clinical Oncology

, Volume 141, Issue 7, pp 1195–1203 | Cite as

Epigenetically silenced long noncoding-SRHC promotes proliferation of hepatocellular carcinoma

Original Article – Cancer Research

Abstract

Purpose

To investigate the expression of SRHC and the role of SRHC in the pathogenesis of hepatocellular carcinoma (HCC).

Methods

We analyzed HCC samples and matched non-tumor liver tissues (controls) collected from 81 patients who underwent hepatectomy in Shanghai, China. The expression levels of SRHC were determined by quantitative reverse-transcription polymerase chain reaction. Statistical analyses were used to associate the levels of SRHC with tumor features and patient outcomes.

Results

We found that a lower SRHC expression level was significantly more frequent in tissues with a high serum a-fetoprotein level (positive, >20 µg/L, P = 0.004) and a low degree of differentiated tumors (poorly differentiated, P = 0.017). Furthermore, we found that the promoter region of SRHC contains a CpG-rich island and that SRHC is down-regulated in tumors by DNA methylation.

Conclusion

Here, we identified a new long noncoding RNA designated as SRHC that is capable of inhibiting cancer proliferation and is down-regulated in tumors at least partly by DNA methylation.

Keywords

Hepatocellular carcinoma Splicing SRHC LncRNA Methylation 

Notes

Acknowledgments

The authors acknowledge Shuai Li for his detailed bioinformatics analysis of SRHC.

Conflict of interest

We declare that we have no conflict of interest.

Supplementary material

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Supplementary material 1 (TIFF 1082 kb)
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Supplementary material 2 (TIFF 727 kb)
432_2014_1871_MOESM3_ESM.docx (15 kb)
Supplementary material 3 (DOCX 14 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghaiChina
  2. 2.Department of Medical GeneticsSecond Military Medical UniversityShanghaiChina
  3. 3.Department of Laboratory Medicine, Changhai HospitalSecond Military Medical UniversityShanghaiChina

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