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Overexpression of CK20, MAP3K8 and EIF5A correlates with poor prognosis in early-onset colorectal cancer patients

  • Berrin Tunca
  • Gulcin Tezcan
  • Gulsah Cecener
  • Unal Egeli
  • Abdullah Zorluoglu
  • Tuncay Yilmazlar
  • Secil Ak
  • Omer Yerci
  • Ersin Ozturk
  • Gorkem Umut
  • Turkkan Evrensel
Original Paper

Abstract

Purpose

Due to ethnic, genetic and environmental factors, the clinical and molecular characteristics of Turkish colorectal cancer (CRC) patients are different from those of Western populations. The aim of this study was to clarify the relevant alterations of gene expression associated with colorectal carcinogenesis in early-onset patients and to identify specific biomarkers that could provide novel therapeutic molecular targets in this population.

Methods

The expression profiles of 114 different genes were evaluated using mRNA PCR arrays in 39 tumors and 20 surgical margin tissue samples from 39 sporadic CRC patients diagnosed at less than 50 years of age.

Results

The expression levels of IMPDH2, CK20, MAP3K8 and EIF5A were strongly up-regulated in CRC tissues compared with normal colorectal tissues (p < 0.05). The highly significant expression ratios of CK20, MAP3K8 and EIF5A observed in the colorectal tumors of patients predicted recurrence (p < 0.05). The expression of IMPDH2, CK20, MAP3K8 and EIF5A was significantly higher in the tumors of patients with short median survival (log-rank p value < 0.05). Progression-free survival was also significantly increased in patients with low expression of the EIF5A gene compared with those who exhibited high expression of this gene (log-rank p value < 0.05).

Conclusion

We demonstrated that high CK20, MAP3K8 and EIF5A expression levels were significant prognostic factors for poor overall survival in CRC patients. Further studies and validations are required; these genes may provide novel therapeutic molecular targets for CRC treatment, as well as new directions for the development of anticancer drugs.

Keywords

Colorectal cancer CK20 MAP3K8 EIF5A Poor prognosis Turkish population 

Abbreviations

CRC

Colorectal cancer

FFPE

Formalin-fixed paraffin-embedded

Ct

Threshold cycle

Notes

Acknowledgments

This work was supported by the Science Foundation of Uludag University (Project number; T (U)-2009/6).

Conflict of interest

None.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Berrin Tunca
    • 1
  • Gulcin Tezcan
    • 1
  • Gulsah Cecener
    • 1
  • Unal Egeli
    • 1
  • Abdullah Zorluoglu
    • 2
  • Tuncay Yilmazlar
    • 3
  • Secil Ak
    • 1
  • Omer Yerci
    • 4
  • Ersin Ozturk
    • 3
  • Gorkem Umut
    • 1
  • Turkkan Evrensel
    • 5
  1. 1.Department of Medical Biology, Medical FacultyUludag UniversityBursaTurkey
  2. 2.Department of General SurgeryAcıbadem HospitalBursaTurkey
  3. 3.Department of General Surgery, Medical FacultyUludag UniversityBursaTurkey
  4. 4.Department of Pathology, Medical FacultyUludag UniversityBursaTurkey
  5. 5.Department of Medical Oncology, Medical FacultyUludag UniversityBursaTurkey

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