Journal of Cancer Research and Clinical Oncology

, Volume 139, Issue 6, pp 1033–1042 | Cite as

KLF8 involves in TGF-beta-induced EMT and promotes invasion and migration in gastric cancer cells

  • Hui Zhang
  • Lili Liu
  • Yafang Wang
  • Guohong Zhao
  • Rougang Xie
  • Changhao Liu
  • Xiao Xiao
  • Kaichun Wu
  • Yongzhan Nie
  • Hongbo ZhangEmail author
  • Daiming FanEmail author
Original Paper



Krüppel-like factor 8 (KLF8), a downstream transcription factor of transforming growth factor-β1 (TGF-β1), has a role in tumorigenesis, tumor progress and epithelial-to-mesenchymal transition (EMT) induction. Recent studies mainly focused on its role in breast cancer and hepatocellular carcinoma; however, little is studied in gastric cancer. Here, we aim to explore whether KLF8 is involved in TGF-β1-induced EMT in gastric cancer cells.


Western blot and real-time PCR assays were used to detect the expression of KLF8, E-cadherin and vimentin in gastric cancer cell line SGC7901 treated with or without TGF-β1. The lentivirus-mediated RNA interference technique was used to knock down the expression of KLF8 in gastric cancer cell line SGC7901. In vitro, the ability of cell migration and invasion were measured by transwell and wound healing assays; the cell motility was detected by high content screening assay.


TGF-β1 could induce EMT via down-regulating E-cadherin and up-regulating vimentin expression in gastric cancer cells. Further study found that TGF-β1 could induce KLF8 expression at the protein and mRNA levels in gastric cancer cells (P < 0.05). Western blot and real-time PCR assays found that small interference RNA (siRNA)-mediated KLF8 silence blocked TGF-β1-induced EMT-like transformation and subsequently reversed the loss of E-cadherin and gain of vimentin. In vitro, inhibition of KLF8 decreased TGF-β1-prompted cell migration, invasion and motility.


KLF8, a transcription factor, is involved in TGF-β1-induced EMT in gastric cancer cells and may be a novel therapeutic target for the treatment of gastric cancer.


TGF-β1 KLF8 EMT siRNA Gastric cancer 



Krüppel-like factor 8


Transforming growth factor-β


Epithelial–mesenchymal transition


Small interference RNA


Extra cellular matrix


Complementary DNA


Messenger RNA


Green fluorescent protein


Negative control



Grant sponsor: National Nature Science Foundation of China (Number: 81030044).

Conflict of interest

We have no conflict of interest to be disclosed.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Hui Zhang
    • 1
  • Lili Liu
    • 2
  • Yafang Wang
    • 1
  • Guohong Zhao
    • 1
  • Rougang Xie
    • 3
  • Changhao Liu
    • 1
  • Xiao Xiao
    • 1
  • Kaichun Wu
    • 1
  • Yongzhan Nie
    • 1
  • Hongbo Zhang
    • 1
    Email author
  • Daiming Fan
    • 1
    Email author
  1. 1.State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing HospitalFourth Military Medical UniversityXi’anChina
  2. 2.Department of Oncology, Tangdu HospitalFourth Military Medical UniversityXi’anChina
  3. 3.Institute of NeuroscienceThe Fourth Military Medical UniversityXi’anChina

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