Journal of Cancer Research and Clinical Oncology

, Volume 136, Issue 11, pp 1651–1655 | Cite as

Application of MALDI imaging for the diagnosis of classical Hodgkin lymphoma

  • Kristina Schwamborn
  • René C. Krieg
  • Peggy Jirak
  • German Ott
  • Ruth Knüchel
  • Andreas Rosenwald
  • Axel Wellmann
Original Paper

Abstract

Hodgkin lymphoma (HL) is a distinctive lymphoma subtype characterized by rareness of tumor cells [Hodgkin’s and Reed-Sternberg (HRS) cells in classical HL and lymphocytic and histiocytic cells in lymphocyte predominant HL] as well as the vast majority of the surrounding inflammatory-like cellular infiltrate. Still the onset of this highly variable disease is not completely understood. Proteome analysis can lead to the identification of potential proteins capable of elucidating malignant growth and survival in HL. Especially MALDI imaging could result in pinpointing differentially expressed proteins, which might represent potential marker molecules. In this study, we were able to distinguish between classical Hodgkin lymphoma and lymphadenitis with a sensitivity and specificity of 83.92 and 89.37%, respectively.

Keywords

Hodgkin lymphoma MALDI imaging Proteomics Tissue profiling Pathology 

Notes

Acknowledgments

We are grateful for excellent technical assistance from N. Reulen and S. Uhlig.

Conflict of interest statement

None.

References

  1. Caldwell RL, Caprioli RM (2005) Tissue profiling by mass spectrometry. Mol Cell Proteomics 4:394–401CrossRefPubMedGoogle Scholar
  2. Carvalho CP, Carvalho Mda G, Degrave W et al (2007) Differential protein expression patterns obtained by mass spectrometry can aid in the diagnosis of Hodgkin’s disease. J Exp Ther Oncol 6:137–145PubMedGoogle Scholar
  3. Celis JE, Gromov P (2003) Proteomics in translational cancer research: toward an integrated approach. Cancer Cell 3:9–15CrossRefPubMedGoogle Scholar
  4. Chaurand P, Stoeckli M, Caprioli RM (1999) Direct profiling of proteins in biological tissue sections by MALDI mass spectrometry. Anal Chem 71:5263–5270CrossRefPubMedGoogle Scholar
  5. Chaurand P, Sanders ME, Jensen RA, Caprioli RM (2004) Proteomics in diagnostic pathology. Am J Pathol 165:1057–1068PubMedGoogle Scholar
  6. Cheson BD (2004) What is new in lymphoma? CA Cancer J Clin 54:260–272CrossRefPubMedGoogle Scholar
  7. Crockett DK, Seiler CE III, Elenitoba-Johnson KS, Lim MS (2005) Annotated proteome of a human T-cell lymphoma. J Biomol Tech 16:341–346PubMedGoogle Scholar
  8. Foss HD, Marafioti T, Stein H (2000) Hodgkin’s lymphoma: classification and pathogenesis. Pathologe 21:113–123CrossRefPubMedGoogle Scholar
  9. Fraga M, Forteza J (2007) Diagnosis of Hodgkin’s disease: an update on histopathological and immunophenotypical features. Histol Histopathol 22:923–935PubMedGoogle Scholar
  10. Fujii K, Kondo T, Yokoo H, Yamada T, Matsuno Y, Iwatsuki K, Hirohashi S (2005) Protein expression pattern distinguishes different lymphoid neoplasms. Proteomics 5:4274–4286CrossRefPubMedGoogle Scholar
  11. Ghobrial IM, McCormick DJ, Kaufmann SH et al (2005) Proteomic analysis of mantle-cell lymphoma by protein microarray. Blood 105:3722–3730CrossRefPubMedGoogle Scholar
  12. Hanash S (2003) Disease proteomics. Nature 422:226–232CrossRefPubMedGoogle Scholar
  13. Hansmann ML, Willenbrock K (2002) WHO classification of Hodgkin’s lymphoma and its molecular pathological relevance. Pathologe 23:207–218CrossRefPubMedGoogle Scholar
  14. Küppers R, Rajewsky K, Zhao M (1994) Hodgkin disease: Hodgkin and Reed-Sternberg cells picked from histological sections show clonal immunoglobulin gene rearrangements and appear to be derived from B cells at various stage of development. Proc Natl Acad Sci USA 91:10962–10966CrossRefPubMedGoogle Scholar
  15. Küppers R, Schwering I, Bräuninger A, Rajewsky K, Hansmann ML (2002) Biology of Hodgkin’s lymphoma. Ann Oncol 13(Suppl 1):11–18PubMedGoogle Scholar
  16. Lin Z, Jenson SD, Lim MS, Elenitoba-Johnson KS (2004) Application of SELDI-TOF mass spectrometry for identification of differentially expressed proteins in transformed follicular lymphoma. Mod Pathol 17:670–678CrossRefPubMedGoogle Scholar
  17. Ma Y, Visser L, Roelofsen H et al (2008) Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes. Blood 111:2339–2346CrossRefPubMedGoogle Scholar
  18. Pileri SA, Ascani S, Leoncini L et al (2002) Hodgkin’s lymphoma: the pathologist’s viewpoint. J Clin Pathol 55:162–176CrossRefPubMedGoogle Scholar
  19. Re D, Küppers R, Diehl V (2005) Molecular pathogenesis of Hodgkin’s lymphoma. J Clin Oncol 23:6379–6386CrossRefPubMedGoogle Scholar
  20. Schwamborn K, Krieg RC, Reska M, Jakse G, Knüchel R, Wellmann A (2007) Identifying prostate carcinoma by MALDI-Imaging. Int J Mol Med 20:155–159PubMedGoogle Scholar
  21. Seeley EH, Caprioli RM (2008) Special Feature: Molecular imaging of proteins in tissues by mass spectrometry. Proc Natl Acad Sci USA 105(47):18126–18131CrossRefPubMedGoogle Scholar
  22. Seitz V, Hummel M, Marafioti T, Anagnostopoulos I, Assaf C, Stein H (2000) Detection of clonal T-cell receptor gamma-chain gene rearrangements in Reed-Sternberg cells of classic Hodgkin disease. Blood 95:3020–3024PubMedGoogle Scholar
  23. Stewart BW, Kleihues P (2003) World cancer report. IARC Press, LyonGoogle Scholar
  24. Stoeckli M, Chaurand P, Hallahan DE, Caprioli RM (2001) Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat Med 7:493–496CrossRefPubMedGoogle Scholar
  25. Thomas RK, Re D, Zander T, Wolf J, Diehl V (2002) Epidemiology and etiology of Hodgkin’s lymphoma. Ann Oncol 13(Suppl 4):147–152PubMedGoogle Scholar
  26. Wallentine JC, Kim KK, Seiler CE 3rd et al (2007) Comprehensive identification of proteins in Hodgkin lymphoma-derived Reed-Sternberg cells by LC–MS/MS. Lab Invest 87:1113–1124CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Kristina Schwamborn
    • 1
  • René C. Krieg
    • 1
  • Peggy Jirak
    • 1
  • German Ott
    • 3
  • Ruth Knüchel
    • 1
  • Andreas Rosenwald
    • 2
  • Axel Wellmann
    • 4
  1. 1.Institute of PathologyRWTH Aachen UniversityAachenGermany
  2. 2.Institute of PathologyUniversity of WurzburgWürzburgGermany
  3. 3.Institute of PathologyRobert-Bosch-KrankenhausStuttgartGermany
  4. 4.Institute of Pathology CelleCelleGermany

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