The importance of −460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer

  • Torben F. Hansen
  • Karen-Lise G. Spindler
  • Karen A. Lorentzen
  • Dorte A. Olsen
  • Rikke F. Andersen
  • Jan Lindebjerg
  • Ivan Brandslund
  • Anders Jakobsen
Original Paper

DOI: 10.1007/s00432-009-0714-1

Cite this article as:
Hansen, T.F., Spindler, KL.G., Lorentzen, K.A. et al. J Cancer Res Clin Oncol (2010) 136: 751. doi:10.1007/s00432-009-0714-1

Abstract

Purpose

The present study investigated the functional influence of the single nucleotide polymorphisms (SNPs) −460 C/T and +405 G/C at vascular endothelial growth factor A (VEGF-A), mRNA and protein levels in colorectal cancer (CRC) and normal colorectal tissue.

Methods

Blood and tissue were collected from 113 patients surgically resected for colorectal cancer. SNPs were analysed from genomic DNA by PCR, the VEGF-A gene expression analysis was performed by RT–PCR and protein analysis by ELISA.

Results

The T-allele in the −460 C/T SNP and the C-allele in the +405 G/C SNP were associated with significantly lower VEGF-A protein levels in normal colorectal tissue. There were no differences in protein levels in the malignant tissue according to genotypes. No differences were observed at the gene expression levels either.

Conclusion

The results indicate that the two SNPs have a functional influence on the VEGF-A protein levels in normal colorectal tissue. The possible clinical implications of the findings need further investigation.

Keywords

Colorectal Cancer Polymorphisms Single nucleotide Vascular endothelial growth factor A 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Torben F. Hansen
    • 1
  • Karen-Lise G. Spindler
    • 1
  • Karen A. Lorentzen
    • 1
  • Dorte A. Olsen
    • 1
  • Rikke F. Andersen
    • 1
  • Jan Lindebjerg
    • 1
  • Ivan Brandslund
    • 1
  • Anders Jakobsen
    • 1
  1. 1.Vejle HospitalVejleDenmark

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