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Tumor-infiltrating macrophages can predict favorable prognosis in hepatocellular carcinoma after resection

  • Yi-Wei Li
  • Shuang-Jian Qiu
  • Jia FanEmail author
  • Qiang Gao
  • Jian Zhou
  • Yong-Sheng Xiao
  • Yang Xu
  • Xiao-Ying Wang
  • Jian Sun
  • Xiao-Wu Huang
Original Paper

Abstract

Purpose

To evaluate the prognostic value of tumor-associated macrophages (TAM), individually or synergistically with CD45RO + memory T cells (TM), in hepatocellular carcinoma (HCC) patients following resection.

Methods

The infiltration of TAM and TM was assessed by immunohistochemistry in tissue microarray containing 302 HCC specimens. Correlations between TAM/TM infiltration and clinicopathologic features, disease-free survival (DFS) and overall survival (OS) were statistically analyzed.

Results

High TAM infiltration was associated with both improved DFS (P = 0.0021) and OS (P = 0.0481). Multivariate analysis identified TAM infiltration as independent prognostic factor for DFS (P = 0.004) and OS (P = 0.049). A second analysis clarified the synergistic effect of TAM&TM infiltration for DFS (P = 0.004) and OS (P = 0.040).

Conclusions

Both TAM infiltration alone and concomitant infiltration of TAM&TM are associated with improved DFS/OS, suggesting that TAM could protect HCC patients from recurrence/metastasis and prolong survival by distinct mechanisms.

Keywords

Tumor-associated macrophages (TAM) CD45RO + T cell (TMHepatocellular carcinoma (HCC) Prognosis 

Abbreviations

HCC

Hepatocellular carcinoma

TAM

Tumor-associated macrophages

TM

CD45RO + T cell

TNM

Tumor-node-metastasis

TACE

Transcatheter arterial chemoembolization

OS

Overall survival

DFS

Disease-free survival

AFP

Alpha-fetoprotein

Notes

Acknowledgements

We thank Dr. Yuan Ji and Dr. Hai-Ying Zeng for their expertise in immunohistochemistry. We also thank Shanghai Biochip Co., Ltd. for help in tissue microarrays construction. The key project was supported by the science and technology program of the Chinese Ministry of Education (no. 107039), National Natural Science Foundation of China (no. 30700794).

Supplementary material

432_2008_469_MOESM1_ESM.doc (82 kb)
Electronic supplementary material Appendix Table 5 and 6 (DOC 82 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Yi-Wei Li
    • 1
  • Shuang-Jian Qiu
    • 1
  • Jia Fan
    • 1
    Email author
  • Qiang Gao
    • 1
  • Jian Zhou
    • 1
  • Yong-Sheng Xiao
    • 1
  • Yang Xu
    • 1
  • Xiao-Ying Wang
    • 1
  • Jian Sun
    • 1
  • Xiao-Wu Huang
    • 1
  1. 1.Liver Cancer Institute, Zhong Shan Hospital and Institutes of Biomedical Sciences, Shanghai Medical SchoolFudan UniversityShanghaiPeople’s Republic of China

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