Tumor-infiltrating macrophages can predict favorable prognosis in hepatocellular carcinoma after resection

  • Yi-Wei Li
  • Shuang-Jian Qiu
  • Jia FanEmail author
  • Qiang Gao
  • Jian Zhou
  • Yong-Sheng Xiao
  • Yang Xu
  • Xiao-Ying Wang
  • Jian Sun
  • Xiao-Wu Huang
Original Paper



To evaluate the prognostic value of tumor-associated macrophages (TAM), individually or synergistically with CD45RO + memory T cells (TM), in hepatocellular carcinoma (HCC) patients following resection.


The infiltration of TAM and TM was assessed by immunohistochemistry in tissue microarray containing 302 HCC specimens. Correlations between TAM/TM infiltration and clinicopathologic features, disease-free survival (DFS) and overall survival (OS) were statistically analyzed.


High TAM infiltration was associated with both improved DFS (P = 0.0021) and OS (P = 0.0481). Multivariate analysis identified TAM infiltration as independent prognostic factor for DFS (P = 0.004) and OS (P = 0.049). A second analysis clarified the synergistic effect of TAM&TM infiltration for DFS (P = 0.004) and OS (P = 0.040).


Both TAM infiltration alone and concomitant infiltration of TAM&TM are associated with improved DFS/OS, suggesting that TAM could protect HCC patients from recurrence/metastasis and prolong survival by distinct mechanisms.


Tumor-associated macrophages (TAM) CD45RO + T cell (TMHepatocellular carcinoma (HCC) Prognosis 



Hepatocellular carcinoma


Tumor-associated macrophages


CD45RO + T cell




Transcatheter arterial chemoembolization


Overall survival


Disease-free survival





We thank Dr. Yuan Ji and Dr. Hai-Ying Zeng for their expertise in immunohistochemistry. We also thank Shanghai Biochip Co., Ltd. for help in tissue microarrays construction. The key project was supported by the science and technology program of the Chinese Ministry of Education (no. 107039), National Natural Science Foundation of China (no. 30700794).

Supplementary material

432_2008_469_MOESM1_ESM.doc (82 kb)
Electronic supplementary material Appendix Table 5 and 6 (DOC 82 kb)


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Yi-Wei Li
    • 1
  • Shuang-Jian Qiu
    • 1
  • Jia Fan
    • 1
    Email author
  • Qiang Gao
    • 1
  • Jian Zhou
    • 1
  • Yong-Sheng Xiao
    • 1
  • Yang Xu
    • 1
  • Xiao-Ying Wang
    • 1
  • Jian Sun
    • 1
  • Xiao-Wu Huang
    • 1
  1. 1.Liver Cancer Institute, Zhong Shan Hospital and Institutes of Biomedical Sciences, Shanghai Medical SchoolFudan UniversityShanghaiPeople’s Republic of China

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