Journal of Cancer Research and Clinical Oncology

, Volume 135, Issue 1, pp 107–116 | Cite as

Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma

  • Salvatore De Maria
  • Giuseppe Pannone
  • Pantaleo Bufo
  • Angela Santoro
  • Rosario Serpico
  • Salvatore Metafora
  • Corrado Rubini
  • Daniela Pasquali
  • Silvana M. Papagerakis
  • Stefania Staibano
  • Gaetano De Rosa
  • Ernesto Farina
  • Monica Emanuelli
  • Andrea Santarelli
  • Maria Ada Mariggiò
  • Lucio Lo Russo
  • Lorenzo Lo Muzio
Original Paper

Abstract

Purpose

Survivin, an inhibitor of apoptosis protein and a cell cycle regulator, has been detected in the majority of human cancers. Five splice variants (survivin, survivin-2α, survivin-2B, survivin-3B, and survivin-ΔEx3) have been identified; their expressions have been investigated here.

Methods

By means of RT real-time PCR and immunohistochemistry, we have evaluated survivin isoform expressions at both mRNA and protein levels in human normal oral tissue, precancerous lesions, and oral squamous cell carcinoma (OSCC). Their correlations with the pathological findings have also been analyzed.

Results

Expression levels of all survivin transcript variants were markedly elevated in OSCC when compared to normal tissues. One-way analysis of variance (ANOVA) revealed highly significant up-regulation of survivin (P = 0.001), survivin-ΔEx3 (P = 0.001) and survivin-2B (= 0.004), whereas survivin-3B showed a minor increase in OSCC compared to normal mucosa.

Conclusions

Our findings suggest that survivin isoforms deregulation may have significant implications in tumor aggressiveness and prognosis.

Keywords

Survivin Apoptosis Splicing isoforms OSCC Dysplasia 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Salvatore De Maria
    • 1
    • 2
  • Giuseppe Pannone
    • 3
  • Pantaleo Bufo
    • 3
  • Angela Santoro
    • 3
  • Rosario Serpico
    • 2
  • Salvatore Metafora
    • 1
  • Corrado Rubini
    • 4
  • Daniela Pasquali
    • 5
  • Silvana M. Papagerakis
    • 6
  • Stefania Staibano
    • 7
  • Gaetano De Rosa
    • 7
  • Ernesto Farina
    • 2
  • Monica Emanuelli
    • 8
  • Andrea Santarelli
    • 3
  • Maria Ada Mariggiò
    • 9
  • Lucio Lo Russo
    • 3
  • Lorenzo Lo Muzio
    • 3
    • 10
  1. 1.Department of Experimental MedicineSecond University of NaplesNaplesItaly
  2. 2.Department of Odontostomatological, Orthodontical and Surgical DisciplinesSecond University of NaplesNaplesItaly
  3. 3.Department of Surgical SciencesUniversity of FoggiaFoggiaItaly
  4. 4.Institute of PathologyUniversity of AnconaAnconaItaly
  5. 5.Department of Clinical and Experimental Medicine and Surgery, Endocrine UnitSecond University of NapolesNaplesItaly
  6. 6.Department of Dental Diagnostic Science, Oral Medicine DivisionUTHSCSASan AntonioUSA
  7. 7.Department of Biomorphological and Functional Sciences, Section of PathologyUniversity of Naples Federico IINaplesItaly
  8. 8.Department of BiochemistryUniversity of AnconaAnconaItaly
  9. 9.Department of Biomedical Sciences and Human Oncology, Section of General Pathology and Experimental OncologyUniversity of BariBariItaly
  10. 10.FoggiaItaly

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