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Journal of Cancer Research and Clinical Oncology

, Volume 134, Issue 9, pp 1021–1027 | Cite as

B7-H1 up-regulated expression in human pancreatic carcinoma tissue associates with tumor progression

  • Lei Geng
  • Dongsheng Huang
  • Junwei Liu
  • Yigang Qian
  • Junfang Deng
  • Donglin Li
  • Zhenhua Hu
  • Jian Zhang
  • Guoping Jiang
  • Shusen ZhengEmail author
Original Paper

Abstract

Purpose

Aberrant tumor cell B7-H1 expression, a member of B7 family that can predominantly stimulate interleukin 10 (IL-10) products, contributed to the tumor immune evasion and tumor progression. This study was designed to investigate the expression of B7-H1 and IL-10 in normal pancreas tissues and pancreatic carcinoma samples, and to evaluate clinical significance of B7-H1 expression in pancreatic carcinoma.

Methods

First, the B7-H1 and IL-10 expression in 40 pancreatic carcinoma samples and 8 healthy pancreas specimens using reverse transcription-PCR (RT-PCR) and western-blotting was detected. Localization of B7-H1 and IL-10 was confirmed by immunohistochemical (IHC) staining. Next, the association between B7-H1 expression and tumor differentiation and tumor stage was analyzed. Finally, the correlation between tumor-associated B7-H1 and IL-10 was evaluated.

Results

Pancreatic carcinoma samples demonstrated the up-regulated expression of B7-H1 and IL-10 at mRNA and protein level compared with normal pancreas tissues. IHC staining revealed that B7-H1 and IL-10 was almost localized in tumor cells. Analysis of relationship between B7-H1 and tumor clinicopathological characteristics showed that B7-H1 expression was significantly associated with poor tumor differentiation (P < 0.01) and advanced tumor stage (P < 0.01). Meanwhile, tumor-associated B7-H1 expression was also correlated with IL-10 products (P < 0.01, R 2 = 0.6985, mRNA level; P < 0.01, R 2 = 0.7236, protein level) in tumor cells.

Conclusions

Our findings for the first time demonstrated up-regulated B7-H1 expression in human pancreatic carcinoma tissues, which might play a role in tumor progression and invasiveness. This expression seemed to be related to the ability of B7-H1 to promoting IL-10 secretion.

Keywords

B7-H1 Pancreatic carcinoma Interleukin-10 

Abbreviations

BSA

Bovine serum albumin

IL-10

Interleukin-10

IHC

Immunohistochemical

MDC

Myeloid dendritic cells

PD-1

Programmed death 1

RT-PCR

Reverse transcription-PCR

TNM

Tumor node metastasis

Notes

Acknowledgments

This work was supported by grants from the National Key Basic Research Program of China (No.2003CB515501) and Science and Technology Department of Zhejiang Province (No. 2006C33027).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Lei Geng
    • 1
  • Dongsheng Huang
    • 2
  • Junwei Liu
    • 2
  • Yigang Qian
    • 1
  • Junfang Deng
    • 1
  • Donglin Li
    • 1
  • Zhenhua Hu
    • 1
  • Jian Zhang
    • 3
  • Guoping Jiang
    • 1
  • Shusen Zheng
    • 1
    Email author
  1. 1.Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health and Department of Hepato-Biliary-Pancreatic SurgeryFirst Affiliated Hospital, Zhejiang University School of MedicineHangzhouChina
  2. 2.Department of General SurgerySir Run Run Shaw Affiliated Hospital, Zhejiang University School of MedicineHangzhouChina
  3. 3.Department of AnesthesiologyFirst Affiliated Hospital, Zhejiang University School of MedicineHangzhouChina

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