Journal of Cancer Research and Clinical Oncology

, Volume 134, Issue 9, pp 1037–1042 | Cite as

Prognostic value of Bmi-1 oncoprotein expression in NSCLC patients: a tissue microarray study

  • Katerina Vrzalikova
  • Joseph Skarda
  • Jiri Ehrmann
  • Paul G. Murray
  • Eduard Fridman
  • Jury Kopolovic
  • Petra Knizetova
  • Marian Hajduch
  • Jiri Klein
  • Vitezslav Kolek
  • Lenka Radova
  • Zdenek Kolar
Original Paper

Abstract

Purpose

Bmi-1 is a Polycomb group member which participates in many physiological processes as well as in a wide spectrum of cancers. The aim of this study was to investigate Bmi-1 expression in non-small cell lung cancer (NSCLC) in respect to clinicopathological features and therapeutic outcomes.

Methods

Immunohistochemical staining for Bmi-1 was performed on tissue microarrays (TMAs) constructed from 179 formalin-fixed and paraffin-embedded NSCLC samples (106 squamous, 58 adeno-, and 15 large cell carcinomas). Data were subject to statistical analysis by SPSS.

Results

Overall evaluation of all tumor cases showed that 20 (11.43%) were negative, 37 (21.14%) showed weak, 65 (37.14%) moderate and 57 (32.57%) strong nuclear positivity for Bmi-1. Statistical analysis of our data revealed that the expression of Bmi-1 was significantly higher in stage III (P = 10−6) and stage IV (P = 10−5) tumors compared to stages I and II tumors. The administration of adjuvant chemotherapy significantly increased DFS at stage I and II patients who did not express Bmi-1 when compared to their Bmi-1 positive counterparts (P = 0.05).

Conclusions

Our results suggest that Bmi-1 is significantly associated with progression of NSCLC and might serve as a prognostic marker of adverse disease outcome.

Keywords

Tissue microarray Non-small cell lung cancer Bmi-1 oncogene Polycomb genes 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Katerina Vrzalikova
    • 1
  • Joseph Skarda
    • 1
  • Jiri Ehrmann
    • 1
  • Paul G. Murray
    • 2
  • Eduard Fridman
    • 3
  • Jury Kopolovic
    • 3
  • Petra Knizetova
    • 1
  • Marian Hajduch
    • 4
  • Jiri Klein
    • 5
  • Vitezslav Kolek
    • 6
  • Lenka Radova
    • 7
  • Zdenek Kolar
    • 1
  1. 1.Laboratory of Molecular Pathology, Department of PathologyPalacky UniversityOlomoucCzech Republic
  2. 2.Cancer Research UK, Institute for Cancer StudiesUniversity of BirminghamBirminghamUK
  3. 3.Department of Pathology, The Chaim Sheba Medical Center and Sackler School of MedicineTel Aviv UniversityTel-AvivIsrael
  4. 4.Laboratory of Experimental Medicine, Department of PediatricsPalacky UniversityOlomoucCzech Republic
  5. 5.1st Department of Surgery, Medical FacultyPalacky UniversityOlomoucCzech Republic
  6. 6.Department of Tuberculosis and Respiratory Diseases, Faculty of Medicine and DentistryPalacky UniversityOlomoucCzech Republic
  7. 7.Department of Pediatrics, Medical FacultyPalacky UniversityOlomoucCzech Republic

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