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Common single nucleotide polymorphisms in the vascular endothelial growth factor gene and colorectal cancer risk

  • Guenter Hofmann
  • Uwe Langsenlehner
  • Wilfried Renner
  • Tanja Langsenlehner
  • Babak Yazdani-Biuki
  • Heimo Clar
  • Armin Gerger
  • Martin Wehrschuetz
  • Hellmut Samonigg
  • Peter Krippl
Original Paper

Abstract

Purpose

Tumor growth requires the formation of new blood vessels, a phenomenon known as angiogenesis. The most important regulator of angiogenesis is vascular endothelial growth factor (VEGF). Several common polymorphisms in the VEGF-gene have been associated with different VEGF expression, production and plasma levels according to allele status, and influence the risk of developing different types of cancer. Therefore, these variants might be risk factors for colorectal cancer (CRC).

Methods

In the present case–control study, VEGF genotypes of the +936 C > T, −2578 C > A and −634 G > C polymorphisms were determined in 427 patients with histologically verified CRC and 427 age and sex-matched healthy control subjects. Genotypes were analyzed by a fluorogenic exonuclease assay (TaqMan™). P-value for age at diagnosis was analyzed by student’s t test, P-values for tumor characteristics were determined by Pearson’s Chi-square test. Threshold for significance was P < 0.05.

Results

At the time of diagnoses, patients were between 29 and 83 years of age, with a mean age of 61 ± 10.9 years. VEGF −2578 C > A and VEGF −634 G > C genotype frequencies were similar among patients and controls. Carriers of the 936T-allele were found slightly more frequent among controls (27.2%) than among patients (22.5%), but this difference did not reach statistical significance (P = 0.07). Furthermore, no correlation was found between all these variants and tumor characteristics like size, histological grading, positive regional lymph node metastases or tumor stage.

Conclusion

We conclude that the investigated polymorphisms are not associated with individual susceptibility to colorectal cancer.

Keywords

Colorectal Cancer Susceptibility VEGF Polymorphisms 

Notes

Acknowledgments

This study was supported by the “Austrian Cancer Aid/Styria” (project no. 02/2005)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Guenter Hofmann
    • 1
  • Uwe Langsenlehner
    • 1
  • Wilfried Renner
    • 2
  • Tanja Langsenlehner
    • 3
  • Babak Yazdani-Biuki
    • 4
  • Heimo Clar
    • 5
  • Armin Gerger
    • 1
  • Martin Wehrschuetz
    • 6
  • Hellmut Samonigg
    • 1
  • Peter Krippl
    • 7
  1. 1.Division of Oncology, Department of Internal MedicineMedical University of GrazGrazAustria
  2. 2.Clinical Institute of Medical and Chemical Laboratory DiagnosticsMedical University of GrazGrazAustria
  3. 3.Department of Therapeutic Radiology and OncologyMedical University of GrazGrazAustria
  4. 4.Division of Rheumatology, Department of Internal MedicineMedical University of GrazGrazAustria
  5. 5.Department of Orthopedic SurgeryMedical University of GrazGrazAustria
  6. 6.Division of General Radiological Diagnostics, Department of RadiologyMedical University of GrazGrazAustria
  7. 7.Department of Internal MedicineRegional Hospital of FuerstenfeldFuerstenfeldAustria

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