Journal of Cancer Research and Clinical Oncology

, Volume 134, Issue 3, pp 365–372

Ecto-5′-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells

  • Li Wang
  • Xuerui Zhou
  • Tingting Zhou
  • Dong Ma
  • Sifeng Chen
  • Xiuling Zhi
  • Lianhua Yin
  • Zhimin Shao
  • Zhouluo Ou
  • Ping Zhou
Original Paper

Abstract

Purpose

Associated with many molecules, metastasis includes cell adhesion to extracellular matrix, migration towards specific direction and invasion into local vessel of distant organs. The purpose of the present study was to evaluate the role of ecto-5′-nucleotidase (eN, ecto-5-NT, CD73) generated extracellular adenosine in biologically malignant behaviors of human breast cancer cell lines.

Materials and methods

Two human breast cancer cell lines, T-47D with lower expression of CD73 and MB-MDA-231 with higher expression of CD73, were used to investigate the functions of CD73. The effects of CD73 over-expression on invasion, migration and adhesion were observed in T-47D transfected with pcDNA-NT5E plasmid. The effects of specific CD73 inhibitor, α, ß-methylene ADP (APCP), were observed in MB-MDA-231 cells.

Results

The results showed CD-73 overexpression increased invasion, migration and adhesion to ECM of the pcDNA-NT5E transfected T-47D cells compared to the saline and mock vector controls. The increased cell mobility of CD-73-overexpressed T-47D cells was blocked by APCP. Adenosine increased the mobility of wild type T-47D cells. APCP inhibited the mobility of the MB-MDA-231 cells.

Conclusion

Taken together, our results indicated that CD73 may facilitate the adhesion, migration and invasion of human breast cancer cells through its enzyme activity of generating adenosine. This study provided a possibly molecular mechanism of metastasis of breast carcinoma.

Keywords

CD73 Adenosine Breast cancer Invasion Adhesion Migration 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Li Wang
    • 1
  • Xuerui Zhou
    • 2
  • Tingting Zhou
    • 1
  • Dong Ma
    • 1
  • Sifeng Chen
    • 1
  • Xiuling Zhi
    • 1
  • Lianhua Yin
    • 1
  • Zhimin Shao
    • 3
  • Zhouluo Ou
    • 3
  • Ping Zhou
    • 1
  1. 1.Department of Physiology and PathophysiologyShanghai Medical College, Fudan UniversityShanghaiPeople’s Republic of China
  2. 2.Department of BiologyHuaiyin Teachers CollegeHuaianPeople’s Republic of China
  3. 3.Department of Breast SurgeryCancer Hospital, Fudan UniversityShanghaiPeople’s Republic of China

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