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Journal of Cancer Research and Clinical Oncology

, Volume 134, Issue 2, pp 179–186 | Cite as

Down-regulation of β-centractin might be involved in dendritic cells dysfunction and subsequent hepatocellular carcinoma immune escape: a proteomic study

  • Yong-Qiang Weng
  • Shuang-Jian Qiu
  • Yin-Kun Liu
  • Jia Fan
  • Qiang Gao
  • Zhao-You TangEmail author
Original Paper

Abstract

Aims

Proteomic study was used to clarify the mechanism of hepatocellular carcinoma (HCC) immune escape concerning Dendritic cells (DCs’) dysfunction and their association with HCC invasion.

Methods

Human peripheral blood mononuclear cells (PBMCs) derived DCs from healthy donors were pulsed with soluble cell lysates prepared from different metastatic potential human HCC cell lines. The total protein of these DCs was analyzed by two-dimensional electrophoresis and Electro-Spray Mass Spectrometry. The allostimulatoy capacity and phenotype of these DCs were also evaluated. The clinical significance of β-centractin, one of the largest quantitative changed spot, down-regulation in DCs was further evaluated in autologous PBMCs derived DCs pulsed with auto-tumor lysates in 26 HCC patients.

Results

The expression of β-centractin was found to be considerably lower either in DCs pulsed with HCCLM6 (high metastatic potential HCC cell line) lysates, accompanied by down-regulation of CD86 molecule and impaired allostimulatory capacity, than those of DCs pulsed with lysates from HCC cell lines with low or without metastatic potential or in DCs pulsed with lysates from HCC with invasiveness than those without invasiveness.

Conclusions

The down-regulation of β-centractin in DCs pulsed with high metastatic potential HCC lysates might associate with DCs dysfunction and HCC invasiveness.

Keywords

Hepatocellular carcinoma (HCC) Dendritic cells (DCs) β-centractin Proteomics 

Abbreviations

HCC

Hepatocellular carcinoma

DCs

Dendritic cell

PBMCs

Peripheral blood mononuclear cells

AFP

Alpha-fetoprotein

APC

Antigen presenting cell

TCR

T-cell receptor

Notes

Acknowledgments

This work was supported by a grant from the National Natural Science Foundation of China (No. 30200268). We thank Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences for helping with the Proteomic analysis, and Shanghai Fudan-Yueda Bio-Tech Co. Ltd., for helping with Western blot analysis of β-centractin expression.

Supplementary material

432_2007_267_MOESM1_ESM.doc (40 kb)
(DOC 39 kb)

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yong-Qiang Weng
    • 1
  • Shuang-Jian Qiu
    • 1
  • Yin-Kun Liu
    • 1
  • Jia Fan
    • 1
  • Qiang Gao
    • 1
  • Zhao-You Tang
    • 1
    Email author
  1. 1.Liver Cancer Institute and Zhong Shan HospitalFudan University, Key Laboratory for Carcinogenesis and Cancer Invasion, Ministry of EducationShanghaiPeople’s Republic of China

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