Journal of Cancer Research and Clinical Oncology

, Volume 133, Issue 10, pp 771–782 | Cite as

Differential proteomic analysis of human colorectal carcinoma cell lines metastasis-associated proteins

  • Liang Zhao
  • Li Liu
  • Shuang Wang
  • Yan-fei Zhang
  • Li Yu
  • Yan-qing Ding
Original Paper

Abstract

Purpose

Metastasis is a common phenomenon and the major lethal cause of colorectal carcinoma (CRC). To better comprehend the mechanism underlying CRC metastasis and to search for potential markers for predicting CRC metastasis, two CRC cell lines with different metastatic potentials, SW480 and SW620, were investigated by phenotypic analyses and proteomics technologies.

Methods

The surgical orthotopic implantation (SOI) technique was originally used to develop a reproducible colorectal cancer model in nude mice with stable tumor growth and metastasizing course. Furthermore, differential proteome analysis of two CRC cell lines was conducted using two-dimensional (2-D) gel electrophoresis combined with matrix-assisted laser desorption/time of flight (MALDI-TOF) mass spectrometry.

Results

Among the differential spots, 12 protein spots (11 proteins) were further identified using in-gel tryptic digestion and peptide mass fingerprinting (PMF). Interestingly, most of these proteins we identified have been reported to be associated with distinct aspect of tumor metastasis to some extent. Our immunohistochemistry assays of colorectal cancer revealed that heat shock protein (HSP) 27 overexpression relates to metastatic behavior of CRC cell.

Conclusions

The protein profile between two colorectal cell lines with different potential metastasis displayed obvious differences. The results imply that various different proteins may lead to CRC metastasis together. HSP27 overexpression played an important role in metastasis and progression of CRC.

Keywords

Surgical orthotopic implantation Colorectal carcinoma Mass spectrometry Metastasis Proteome Two-dimensional gel electrophoresis Heat shock protein 

Abbreviations

2-DE

Two-dimensional electrphoresis

CPOX

Coproporphyrinogen III oxidase

CRC

Colorectal carcinoma

E-FABP

Epidermal fatty acid binding protein

HMGB

High-mobility group box

HSP

Heat shock protein

MTAP

Methylthioadenosine phosphorylase

PBP

Posphatidylethanolamine binding protein

PGAM

Phosphoglycerate mutase

MALDI-TOF

Matrix-assisted laser desorption-ionization-time of flight

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Liang Zhao
    • 1
    • 2
    • 3
  • Li Liu
    • 1
  • Shuang Wang
    • 2
    • 3
  • Yan-fei Zhang
    • 2
  • Li Yu
    • 4
  • Yan-qing Ding
    • 1
    • 2
    • 3
  1. 1.Department of Pathology, Nanfang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Department of Pathology, School of Basic Medical SciencesSouthern Medical UniversityGuangzhouChina
  3. 3.Key Laboratory of Functional Proteomics and Molecular Pathology of Guangdong ProvinceSouthern Medical UniversityGuangzhouChina
  4. 4.Department of Pathology, Zhujiang HospitalSouthern Medical UniversityGuangzhouChina

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