Journal of Cancer Research and Clinical Oncology

, Volume 132, Issue 2, pp 92–98 | Cite as

Proteomic analysis on metastasis-associated proteins of human hepatocellular carcinoma tissues

  • Hai-Yan Song
  • Yin-Kun Liu
  • Ju-Tao Feng
  • Jie-Feng Cui
  • Zhi Dai
  • Li-Jun Zhang
  • Jiu-Xian Feng
  • Hua-Li Shen
  • Zhao-You Tang
Original Paper

Abstract

Purpose: A comparative proteomic approach was used to identify and analyze proteins related to metastasis of hepatocellular carcinoma (HCC). Methods: Proteins extracted from 12 HCC tissue specimens (six with metastases and six without) were separated by two-dimensional gel electrophoresis (2-DE). The protein spots exhibiting statistical alternations between the two groups through computerized image analysis were then identified by mass spectrometry. In addition immunohistochemistry (IHC), Western blotting and RT-PCR were performed to verify the expression of certain candidate proteins. Results: 16 proteins including HSP27, S100A11, CK18 were annotated by mass spectrometry, relevant to chaperone function, cell mobility, cytoskeletal architecture, respectively. Most were previously unconnected with metastasis of HCC. Of these HSP27 was found overexpressed consistently in 2-DE patterns of all metastatic HCC tissues compared with nonmetastatic ones. IHC and Western blotting of HCC tissues confirmed this difference while RT-PCR did not. Conclusion: There are various proteins joined together in HCC metastasis. The overexpression of HSP27 may serve as a biomarker for early detection and therapeutic targets unique to the metastatic phenotype of HCC.

Keywords

Human hepatocellular carcinoma Metastasis Two-dimensional gel electrophoresis Heat shock protein 27 

Abbreviations

IHC

Immunohistochemistry

RT-PCR

Reverse transcription polymerase chain reaction

MALDI-TOF MS

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Hai-Yan Song
    • 1
  • Yin-Kun Liu
    • 1
  • Ju-Tao Feng
    • 1
  • Jie-Feng Cui
    • 1
  • Zhi Dai
    • 1
  • Li-Jun Zhang
    • 2
  • Jiu-Xian Feng
    • 3
  • Hua-Li Shen
    • 4
  • Zhao-You Tang
    • 1
  1. 1.Liver Cancer Institute, Zhongshan HospitalFudan UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Molecular Genetics, Shanghai Medical CollegeFudan UniversityShanghaiPeople’s Republic of China
  3. 3.Chest HospitalShanghaiPeople’s Republic of China
  4. 4.Department of ChemistryFudan UniversityShanghaiPeople’s Republic of China

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