European Journal of Pediatrics

, Volume 174, Issue 5, pp 577–582 | Cite as

Midstream clean-catch urine collection in newborns: a randomized controlled study

  • Nilgun AltuntasEmail author
  • Asli Celebi Tayfur
  • Mesut Kocak
  • Hasan Cem Razi
  • Serpil Akkurt
Original Article


We aimed to evaluate a recently defined technique based on bladder stimulation and paravertebral lumbar massage maneuvers in collecting a midstream clean-catch urine sample in newborns. A total of 127 term newborns were randomly assigned either to the experimental group or the control group. Twenty-five minutes after feeding, the genital and perineal areas of the babies were cleaned. The babies were held under the armpits with legs dangling. Bladder stimulation and lumbar paravertebral massage maneuvers were only applied to the babies in the experimental group. Success was defined as collection of a urine sample within 5 min of starting the stimulation maneuvers in the experimental group and of holding under the armpits in the control group. The success rate of urine collection was significantly higher in the experimental group (78 %) than in the control group (33 %; p < 0.001). The median time (interquartile range) for sample collection was 60 s (64.5 s) in the experimental group and 300 s (95 s) in the control group (p < 0.0001). Contamination rates were similar in both groups (p = 0.770).

Conclusion: We suggest that bladder stimulation and lumbar paravertebral massage is a safe, quick, and effective way of collecting midstream clean-catch urine in newborns.


Clean-catch midstream urine Contamination Midstream urine sample Newborn 



The control group


Clean-catch urine


The experimental group


Sterile bag urine


Suprapubic aspiration


Urine catheterization


Urinary tract infection


Colony-forming units


Funding source

No funding was secured for this study.

Financial disclosure

The authors have no financial relationships relevant to this article to disclose.

Conflict of interest

The authors have no conflicts of interest to disclose.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Nilgun Altuntas
    • 1
    Email author
  • Asli Celebi Tayfur
    • 2
  • Mesut Kocak
    • 3
  • Hasan Cem Razi
    • 3
  • Serpil Akkurt
    • 3
  1. 1.Department of Pediatrics, Division of NeonatologyKecioren Training and Research HospitalKeciorenTurkey
  2. 2.Department of Pediatrics, Division of NephrologyKecioren Training and Research HospitalAnkaraTurkey
  3. 3.Department of PediatricsKecioren Training and Research HospitalAnkaraTurkey

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