European Journal of Pediatrics

, Volume 171, Issue 6, pp 941–946 | Cite as

Urinary matrix metalloproteinases-2/9 in healthy infants and haemangioma patients prior to and during propranolol therapy

  • C. J. Kleber
  • A. Spiess
  • J. B. Kleber
  • U. Hinz
  • S. Holland-Cunz
  • J. Weiss
Original Article

Abstract

The mechanism of therapeutic success of propranolol for severe infantile haemangioma remains unclear. Propranolol was shown to modify matrix metalloproteinase (MMP) levels, which are associated with tumour pathogenesis. We hypothesized that urinary MMP2/9 is higher in patients with infantile haemangioma compared to healthy infants and that propranolol reduces MMP2/9 levels and thus leads to an involution of the haemangioma. In this case, MMP2/9 could be used as a marker of indicated therapy or therapeutic success. Urinary samples were taken before, 2 weeks after, and 2 months after the beginning of propranolol treatment in haemangioma patients and once in healthy controls. Activity of MMP2/9 was determined by commercially available activity kits. Urine of 22 haemangioma patients and 21 control subjects was obtained. Propranolol therapy had significant success in all patients. MMP2/9 was present in most samples, the younger the children the higher the MMP2 levels. Haemangioma patients showed lower levels of MMP2. The MMP2 levels were significantly higher after 2 weeks of propranolol than prior to therapy. There were no differences in MMP9 levels. Conclusions: Presence of MMP2/9 in the urine of infants <1 year can be explained by high rate of physiological tissue remodelling. Unexpectedly, MMP2 was lower in the urine of haemangioma patients and higher 2 weeks after propranolol treatment. Taking this and the diverse results in literature into account, the correlation between MMPs, proliferation, and regression of haemangiomas and propranolol remains unclear.

Keywords

Haemangioma Propranolol Matrix metalloproteinases 

Notes

Acknowledgements

The study was funded by the Heidelberg Foundation of Surgery. We thank the paediatric offices of Dr. Wiens and Drs. Scheffzek and Greiner for their kind help with sample collection.

Conflict of interest

No conflicts of interest for any author. No financial relationship of any author with the Heidelberg Foundation of Surgery.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • C. J. Kleber
    • 1
  • A. Spiess
    • 1
    • 2
  • J. B. Kleber
    • 3
  • U. Hinz
    • 4
  • S. Holland-Cunz
    • 1
  • J. Weiss
    • 2
  1. 1.Devision of Paediatric SurgeryUniversity Hospital HeidelbergHeidelbergGermany
  2. 2.Department of Clinical Pharmacology and PharmacoepidemiologyUniversity Hospital HeidelbergHeidelbergGermany
  3. 3.Devision of Paediatric CardiologyUniversity Hospital HeidelbergHeidelbergGermany
  4. 4.Department of General, Visceral and Transplantation Surgery Unit for Documentation and StatisticsUniversity Hospital HeidelbergHeidelbergGermany

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