European Journal of Pediatrics

, Volume 167, Issue 12, pp 1427–1434 | Cite as

Outbreaks of human coronavirus in a paediatric and neonatal intensive care unit

  • Arnaud Gagneur
  • Sophie Vallet
  • Pierre J. Talbot
  • Marie-Christine Legrand-Quillien
  • Bertrand Picard
  • Christopher Payan
  • Jacques Sizun
Original Paper

Abstract

Human coronavirus 229E (HCoV) has been recently recognized as a potential agent of nosocomial viral respiratory infections (NRVI) in high-risk infants. We have confirmed this as fact through the study of a 1-year period of HCoV outbreaks occurring during a prospective survey of NRVI in a paediatric and neonatal intensive care unit (PNICU) using new molecular techniques for HCoV detection. Nasal samples obtained at admission and weekly thereafter for all hospitalised children, as well as monthly nasal samples from staff, were analysed using immunofluorescence for respiratory syncitial virus (RSV), influenza viruses A and B, paramyxoviruses 1, 2, 3 and adenoviruses. RT-PCR was used for HCoV detection. During the year 1998, 43 HCoV-related NRVI were detected in 152 neonates (incidence 28.3%), and 7 HCoV-related NRVI were found in 92 children (incidence 7.6%). Three HCoV-related outbreaks were observed (February, August and December), associated with a high prevalence of HCoV infection in the staff. During the August outbreak, 50% to 78% of hospitalised neonates and children were infected. Seventy-five percent of hospitalised preterm neonates with a gestational age less than 32 weeks and 52.4% of staff members were infected. Risk factors for NRVI in neonates were birth weight, gestational age, ventilation, oxygenation and hospitalisation length. Ninety-two percent of infected preterm neonates were symptomatic, mainly with bradycardia and respiratory worsening. These data provide additional evidence for a possibly significant role of HCoV in NRVI in a PNICU. The role of staff or hospitalised children in spreading HCoV is hypothesised.

Keywords

Human coronavirus Outbreak Neonates Nosocomial infection PNICU 

Notes

Acknowledgments

This study was supported in part by research funds from the French Ministry of Health, the CCLIN Ouest and the Société Française de Pédiatrie. We would like to thank Anne Gagneur, Marie-Annick L’Her, Michèle Odermatt and Christine Roger for excellent technical assistance, PNICU staff members and Tracey Montagnon for reviewing the manuscript.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Arnaud Gagneur
    • 1
    • 4
  • Sophie Vallet
    • 2
  • Pierre J. Talbot
    • 3
  • Marie-Christine Legrand-Quillien
    • 2
  • Bertrand Picard
    • 2
  • Christopher Payan
    • 2
  • Jacques Sizun
    • 1
  1. 1.Department of Paediatrics, EA 3882 Laboratory of Biodiversity and Microbial EcologyUniversity HospitalBrestFrance
  2. 2.Department of Microbiology, EA 3882 Laboratory of Biodiversity and Microbial EcologyUniversity HospitalBrestFrance
  3. 3.Laboratory of Neuroimmunovirology, INRS-Institut Armand-FrappierUniversity of QuebecLavalCanada
  4. 4.Unité de Réanimation Pédiatrique et Néonatalogie, Département de Pédiatrie CHUBrestFrance

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