European Journal of Pediatrics

, Volume 167, Issue 1, pp 75–80

Clinical manifestations of human coronavirus NL63 infection in children in Taiwan

  • Ping-Sheng Wu
  • Luan-Yin Chang
  • B. Berkhout
  • L. van der Hoek
  • Chun-Yi Lu
  • Chuan-Liang Kao
  • Ping-Ing Lee
  • Pei-Lan Shao
  • Chin-Yun Lee
  • Fu-Yuan Huang
  • Li-Min Huang
Original Paper

Abstract

Human coronavirus NL63 (HCoV-NL63) is a global respiratory tract pathogen; however, the epidemiology of this virus in subtropical area is not well known. To evaluate the epidemics and disease spectrum of HCoV-NL63 infection in children in Taiwan, we prospectively screened children admitted to the hospital with respiratory tract infection from May 2004 to April 2005. Every enrolled child had a nasopharyngeal aspirate (NPA) sample taken. Quantitative RT-PCR was used to detect 1b gene of HCoV-NL63. A total of 539 NPAs were collected. Seven (1.3%) were positive for HCoV-NL63. All cases were boys younger than 3 years of age and most cases occurred in autumn. Co-infection with other pathogens was observed in three cases. The most common symptoms/signs of HCoV-NL63 infection were cough, fever, and inspiratory stridor. HCoV-NL63 was the most common pathogen (14.7%) in children with croup and was the cause of three cases of croup in October. The odds ratio of croup in children infected with HCoV-NL63 was 43.4 (95% CI 8.1∼233.1). In conclusion, HCoV-NL63 is an important respiratory tract pathogen as the main cause in children admitted to the hospital in Taiwan.

Keywords

Coronavirus infections Respiratory tract infections Croup Taiwan 

References

  1. 1.
    Arden KE, Nissen MD, Sloots TP, Mackay IM (2005) New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia. J Med Virol 75(3):455–462PubMedCrossRefGoogle Scholar
  2. 2.
    Bastien N, Anderson K, Hart L, Van Caeseele P, Brandt K, Milley D, Hatchette T, Weiss EC, Li Y (2005) Human coronavirus NL63 infection in Canada. J Infect Dis 191(4):503–506PubMedCrossRefGoogle Scholar
  3. 3.
    Chiu SS, Chan KH, Chu KW, Kwan SW, Guan Y, Poon LL, Peiris JS (2005) Human coronavirus NL63 infection and other coronavirus infections in children hospitalized with acute respiratory disease in Hong Kong, China. Clin Infect Dis 40(12):1721–1729PubMedCrossRefGoogle Scholar
  4. 4.
    Denny FW, Murphy TF, Clyde WA, Collier AM, Henderson FW (1983) Croup: an 11-year study in a pediatric practice. Pediatrics 71(6):871–876PubMedGoogle Scholar
  5. 5.
    Ebihara T, Endo R, Ma X, Ishiguro N, Kikuta H (2005) Lack of association between New Haven coronavirus and Kawasaki disease. J Infect Dis 192(2):351–352PubMedCrossRefGoogle Scholar
  6. 6.
    Esper F, Weibel C, Ferguson D, Landry ML, Kahn JS (2005) Evidence of a novel human coronavirus that is associated with respiratory tract disease in infants and young children. J Infect Dis 191(4):492–498PubMedCrossRefGoogle Scholar
  7. 7.
    Esper F, Shapiro ED, Weibel C, Ferguson D, Landry ML, Kahn JS (2005) Association between a novel human coronavirus and Kawasaki disease. J Infect Dis 191(4):499–502PubMedCrossRefGoogle Scholar
  8. 8.
    Marx A, Török TJ, Holman RC, Clarke MJ, Anderson LJ (1997) Pediatric hospitalizations for croup (laryngotracheobronchitis): biennial increases associated with human parainfluenza virus 1 epidemics. J Infect Dis 176(6):1423–1427PubMedCrossRefGoogle Scholar
  9. 9.
    Moes E, Vijgen L, Keyaerts E, Zlateva K, Li S, Maes P, Pyrc K, Berkhout B, van der Hoek L, Van Ranst M (2005) A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium. BMC Infect Dis 5(1):6PubMedCrossRefGoogle Scholar
  10. 10.
    Shimizu C, Shike H, Baker SC, Garcia F, van der Hoek L, Kuijpers TW, Reed SL, Rowley AH, Shulman ST, Talbot HK, Williams JV, Burns JC (2005) Human coronavirus NL63 is not detected in the respiratory tracts of children with acute Kawasaki disease. J Infect Dis 192(10):1767–1771PubMedCrossRefGoogle Scholar
  11. 11.
    Suzuki A, Okamoto M, Ohmi A, Watanabe O, Miyabayashi S, Nishimura H (2005) Detection of human coronavirus-NL63 in children in Japan. Pediatr Infect Dis J 24(7):645–646PubMedCrossRefGoogle Scholar
  12. 12.
    Vabret A, Mourez T, Dina J, van der Hoek L, Gouarin S, Petitjean J, Brouard J, Freymuth F (2005) Human coronavirus NL63, France. Emerg Infect Dis 11(8):1225–1229PubMedGoogle Scholar
  13. 13.
    van der Hoek L, Pyrc K, Jebbink MF, Vermeulen-Oost W, Berkhout RJ, Wolthers KC, Wertheim-van Dillen PM, Kaandorp J, Spaargaren J, Berkhout B (2004) Identification of a new human coronavirus. Nat Med 10(4):368–373PubMedCrossRefGoogle Scholar
  14. 14.
    van der Hoek L, Sure K, Ihorst G, Stang A, Pyrc K, Jebbink MF, Petersen G, Forster J, Berkhout B, Uberla K (2005) Croup is associated with the novel coronavirus NL63. PLoS Med 2(8):e240PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Ping-Sheng Wu
    • 1
  • Luan-Yin Chang
    • 2
  • B. Berkhout
    • 5
  • L. van der Hoek
    • 5
  • Chun-Yi Lu
    • 2
  • Chuan-Liang Kao
    • 3
  • Ping-Ing Lee
    • 2
  • Pei-Lan Shao
    • 2
  • Chin-Yun Lee
    • 2
  • Fu-Yuan Huang
    • 4
  • Li-Min Huang
    • 2
  1. 1.Department of PediatricsBuddhist Tzu Chi General Hospital, Taipei BranchTaipeiTaiwan
  2. 2.Department of PediatricsNational Taiwan University HospitalTaipeiTaiwan
  3. 3.Department of Laboratory MedicineNational Taiwan University HospitalTaipeiTaiwan
  4. 4.Department of PediatricsTaipei Mackay Memorial HospitalTaipeiTaiwan
  5. 5.Department of Human Retrovirology, Academic Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands

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