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Medical Microbiology and Immunology

, Volume 189, Issue 4, pp 177–183 | Cite as

Natural antibodies to nematode biotinyl-enzymes in human sera

  • S. Lorenzo
  • R. Iglesias
  • E. Paniagua
  • I. Ansotegui
  • J. Alonso
  • F. Ubeira
Original Investigation

Abstract

Biotinyl-enzymes are conservative molecules present in helminths, as well as in other animals, bacteria and plants. They have recently been found to be antigenic in mice, and a potential source of cross-reactivityamonghelminths. This study investigated the presence in human sera of antibodies reactive with biotinyl-enzymes from the nematodes Anisakis simplex, Toxocara canis and Ascaris suum. Biotinyl-enzymes from all these nematodes were recognized by IgG1 antibodies in sera from healthy subjects and from Anisakis-free patients infected with other parasites. Interestingly, IgE antibodies reactive with Anisakis simplex biotinyl-enzymes were present in about one third of the sera from Anisakis-free patients infected with other parasites. Our results also demonstrate that the anti-BE IgG1 and IgE antibodies present in the sera of Anisakis-free subjects are cross-reactive among helminths. We conclude that biotinyl-enzymes from nematodes are recognized by natural human antibodies, although Anisakis biotinyl-enzymes do not seem to be the cause of sensitization. Since sera from the Anisakis-free population also present these antibodies, as-yet unidentified factors (dietary components, intestinal inflammation and/or the presence of parasites) may contribute to the induction of anti-BE antibody background.

Nematode Anisakis simplex Biotinyl-enzymes Natural antibodies 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • S. Lorenzo
    • 1
  • R. Iglesias
    • 1
  • E. Paniagua
    • 1
  • I. Ansotegui
    • 2
  • J. Alonso
    • 3
  • F. Ubeira
    • 1
  1. 1.Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, SpainSpain
  2. 2.Servicio de Alergología, Hospital Santiago Apóstol, Vitoria-Gasteiz, SpainSpain
  3. 3.Instituto de Medicina Regional, Universidad Nacional del Nordeste, Resistencia, Chaco, ArgentinaArgentina

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