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Medical Microbiology and Immunology

, Volume 185, Issue 4, pp 223–229 | Cite as

Tumor necrosis factor-α expression induced by anti-YopB antibodies coincides with protection against Yersinia enterocolitica infection in mice

  • Susanne Burdack
  • Annette Schmidt
  • Elsbeth Knieschies
  • Martin Röllinghoff
  • H. U. Beuscher
ORIGINAL INVESTIGATION

Abstract

Previous studies have suggested that virulence of pathogenic Yersiniae is associated with a suppression of the local cytokine response. In this context, the plasmid-encoded 41-kDa Yersinia outer protein B (YopB) has been implicated with the lack of tumor necrosis factor-α (TNF-α) expression in Peyer's patches (PP), following oral infection of mice with the enteropathogenic Yersinia enterocolitica. The present study was performed to further evaluate the relationships between YopB-induced suppression of TNF-α and bacterial survival in host tissue. Results are presented to show the ability of purified YopB to suppress the release of TNF-α by macrophages, the effect of which was neutralized by monospecific anti-YopB antiserum. In mice orally infected with Y. enterocolitica, anti-YopB treatment on days 3 and 5 postinfection, significantly decreased the recovery of live bacteria from PP. This observation correlated with a strong increase in TNF-α expression, as determined by reverse transcription-polymerase chain reaction and measuring the levels of TNF activity in homogenates of PP. Moreover, treatment of mice with a combination of anti-YopB and anti-TNF-α antiserum, completely abrogated the beneficial effect of the anti-YopB antiserum. In controls, expression of other proinflammatory cytokines such as interleukin-1 remained unaffected by either treatment. Therefore, the results indicate that endogenous TNF-α is required for eradication of Y. enterocolitica from host tissue, and further imply that YopB significantly contributes to suppression of the local TNF-α response in PP.

Key wordsYersinia enterocolitica YopB Tumor necrosis factor-α Anti-YopB antiserum Infection 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Susanne Burdack
    • 1
  • Annette Schmidt
    • 1
  • Elsbeth Knieschies
    • 1
  • Martin Röllinghoff
    • 1
  • H. U. Beuscher
    • 1
  1. 1.Institut für Klinische Mikrobiologie und Immunologie, Universität Erlangen/Nürnberg, Wasserturmstrasse 3, D-91054 Erlangen, Germany Fax: 49-9131-852573DE

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