Advertisement

Medical Microbiology and Immunology

, Volume 188, Issue 4, pp 177–184 | Cite as

Binding of human lipoproteins (low, very low, high density lipoproteins) to recombinant envelope proteins of hepatitis C virus

  • Masyar Monazahian
  • Serena Kippenberger
  • Anke Müller
  • Holger Seitz
  • Ingo Böhme
  • Stefanie Grethe
  • Reiner Thomssen
ORIGINAL INVESTIGATION

Abstract

Heterogeneities in the density of hepatitis C virus (HCV)-RNA-carrying material from human sera (1.03–1.20 g/ml) are partially due to the binding of lipoproteins [low density (LDL), very low density (VLDL), high density (HDL) lipoproteins] and immunoglobulins. In this study we demonstrate the binding of recombinant HCV envelope protein (E1/E2) to human LDL, VLDL and HDL on a molecular basis. The binding of lipoproteins was restricted to the middle part of the E1 gene product (amino acids 222–336) and the C-terminal part of the E2 protein (amino acids 523–809). Lipoproteins did not bind to recombinant HCV core protein.

Key words Hepatitis C virus Lipoprotein Low, very low, high density lipoprotein Recombinant hepatitis C virus envelope protein Binding site 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Masyar Monazahian
    • 1
  • Serena Kippenberger
    • 1
  • Anke Müller
    • 1
  • Holger Seitz
    • 1
  • Ingo Böhme
    • 1
  • Stefanie Grethe
    • 1
  • Reiner Thomssen
    • 1
  1. 1.Department of VirologyUniversity of GöttingenDeutschland

Personalised recommendations