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Biogenic Au@ZnO core–shell nanocomposites kill Staphylococcus aureus without provoking nuclear damage and cytotoxicity in mouse fibroblasts cells under hyperglycemic condition with enhanced wound healing proficiency

  • Md. Imran Khan
  • Susanta Kumar Behera
  • Prajita Paul
  • Bhaskar Das
  • Mrutyunjay Suar
  • R. Jayabalan
  • Derek Fawcett
  • Gerrard Eddy Jai Poinern
  • Suraj K. Tripathy
  • Amrita Mishra
Original Investigation
  • 37 Downloads

Abstract

The aim of the present study is focused on the synthesis of Au@ZnO core–shell nanocomposites, where zinc oxide is overlaid on biogenic gold nanoparticles obtained from Hibiscus Sabdariffa plant extract. Optical property of nanocomposites is investigated using UV–visible spectroscopy and crystal structure has been determined using X-ray crystallography (XRD) technique. The presence of functional groups on the surface of Au@ZnO core–shell nanocomposites has been observed by Fourier transforms infrared (FTIR) spectroscopy. Electron microscopy studies revealed the morphology of the above core–shell nanocomposites. The synthesized nanocomposite material has shown antimicrobial and anti-biofilm activity against Staphylococcus aureus and Methicillin Resistant Staphylococcus haemolyticus (MRSH). The microbes are notorious cross contaminant and are known to cause infection in open wounds. The possible antimicrobial mechanism of as synthesized nanomaterials has been investigated against Staphylococcus aureus and obtained data suggests that the antimicrobial activity could be due to release of reactive oxygen species (ROS). Present study has revealed that surface varnishing of biosynthesized gold nanoparticles through zinc oxide has improved its antibacterial proficiency against Staphylococcus aureus, whereas reducing its toxic effect towards mouse fibroblast cells under normal and hyperglycaemic condition. Further studies have been performed in mice model to understand the wound healing efficiency of Au@ZnO nanocomposites. The results obtained suggest the possible and effective use of as synthesized core shell nanocomposites in wound healing.

Keywords

Biofilm Core–shell Gold Staphylococcus aureus Methicillin resistant Staphylococcus haemolyticus Zinc oxide 

Notes

Acknowledgements

This work is supported by Department of Biotechnology (DBT), Government of India (Grant No. BT/AB/08/01/2008-III). Md. Imran Khan is thankful to University Grant Commission, New Delhi for supporting him through UGC-MANF programme.

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest related to this article.

Supplementary material

430_2018_564_MOESM1_ESM.docx (48.2 mb)
Supplementary material 1 (DOCX 49336 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Md. Imran Khan
    • 1
  • Susanta Kumar Behera
    • 2
  • Prajita Paul
    • 1
  • Bhaskar Das
    • 3
  • Mrutyunjay Suar
    • 1
  • R. Jayabalan
    • 3
  • Derek Fawcett
    • 4
  • Gerrard Eddy Jai Poinern
    • 4
  • Suraj K. Tripathy
    • 1
    • 5
  • Amrita Mishra
    • 1
  1. 1.School of BiotechnologyKalinga Institute of Industrial Technology (KIIT)BhubaneswarIndia
  2. 2.IMGENEX India Pvt. Ltd.BhubaneswarIndia
  3. 3.Department of Life SciencesNational Institute of TechnologyRourkelaIndia
  4. 4.Murdoch Applied Nanotechnology Research Group, Department of Physics, Energy Studies and Nanotechnology, School of Engineering and EnergyMurdoch UniversityMurdochAustralia
  5. 5.School of Chemical TechnologyKalinga Institute of Industrial Technology (KIIT)BhubaneswarIndia

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