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Medical Microbiology and Immunology

, Volume 207, Issue 5–6, pp 307–318 | Cite as

Cellular distribution of CD200 receptor in rats and its interaction with cytomegalovirus e127 protein

  • Mohamed A. El-Mokhtar
  • Agnieszka Bauer
  • Julia Madela
  • Sebastian Voigt
Original Investigation

Abstract

CD200 is a membrane protein that interacts with CD200R on the surface of immune cells and delivers an inhibitory signal. In this study, we characterized the distribution of inhibitory CD200R in rats. In addition, we investigated if e127, a homologue of rat CD200 expressed by rat cytomegalovirus (RCMV), can suppress immune functions in vitro. RT-PCR analysis was carried out to test the expression of CD200R in different rat tissues and flow cytometry was performed to characterize CD200R at the cellular level. To test the inhibitory functions of e127, a co-culture system was utilized in which immune cells were incubated with e127-expressing cells. The strongest CD200R expression was detected in lymphoid organs such as bone marrow and spleen. Flow cytometry analyses showed that CD200R+ cells were mainly CD4 dendritic cells (DC) and CD4+ T cells in the spleen. In blood, nearly all monocytes and granulocytes expressed CD200R and in bone marrow the NKRP1low subset of natural killer cells highly expressed CD200R. In addition, both peritoneal macrophages and the NR8383 macrophage cell line carried CD200R. At the functional level, viral e127 conferred an inhibitory signal on TNFα and IL6 cytokine release from IFNγ-stimulated macrophages. However, e127 did not affect the cytotoxic activity of DC. CD200R in the rat is mainly expressed on myeloid cells but also on non-myeloid cell subsets, and RCMV e127 can deliver inhibitory signals to immune cells by engaging CD200R. The RCMV model provides a useful tool to study potential immune evasion mechanisms of the herpesviridae and opens new avenues for understanding and controlling herpesvirus infections.

Keywords

Cytomegalovirus e127 protein CD200 receptor Viral CD200 homologues Viral immune evasion 

Notes

Funding

Mohamed A. El-Mokhtar was kindly supported by the GERLS (German Egyptian Research Long-Term Scholarships) program from the German Academic Exchange Service (DAAD).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethics approval

The rats were kept under specific pathogen-free conditions with ad libitum diet at the animal facility of the Robert Koch Institute. All procedures performed involving animals were in accordance with the ethical standards of the Robert Koch Institute, Berlin, Germany, and were approved by the ethics committee of Berlin state authorities.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Infectious DiseasesRobert Koch InstituteBerlinGermany
  2. 2.Department of Pediatric Oncology/Hematology/SCTCharité-UniversitätsmedizinBerlinGermany
  3. 3.Department of Medical Microbiology and Immunology, Faculty of MedicineAssiut UniversityAssiutEgypt

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