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Medical Microbiology and Immunology

, Volume 204, Issue 6, pp 681–692 | Cite as

Prevalence of Mycobacterium avium subsp. paratuberculosis and Escherichia coli in blood samples from patients with inflammatory bowel disease

  • Nair Nazareth
  • Fernando Magro
  • Elisabete Machado
  • Teresa Gonçalves Ribeiro
  • António Martinho
  • Pedro Rodrigues
  • Rita Alves
  • Gonçalo Nuno Macedo
  • Daniela Gracio
  • Rosa Coelho
  • Candida Abreu
  • Rui Appelberg
  • Camila Dias
  • Guilherme Macedo
  • Tim Bull
  • Amélia Sarmento
Original Investigation

Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) and adherent-invasive Escherichia coli (AIEC) have been implicated as primary triggers in Crohn’s disease (CD). In this study, we evaluated the prevalence of MAP and E. coli (EC) DNA in peripheral blood from 202 inflammatory bowel disease (IBD) patients at various disease periods and compared against 24 cirrhotic patients with ascites (CIR) (non-IBD controls) and 29 healthy controls (HC). MAP DNA was detected by IS900-specific nested PCR, EC DNA by malB-specific nested PCR and AIEC identity, in selected samples, by sequencing of fimH gene. CD patients with active disease showed the highest MAP DNA prevalence among IBD patients (68 %). Infliximab treatment resulted in decreased MAP detection. CIR patients had high individual and coinfection rates (75 % MAP, 88 % EC and 67 % MAP and EC), whilst HC controls had lower MAP prevalence (38 %) and EC was undetectable in this control group. EC DNA prevalence in IBD patients was highly associated with CD, and 80 % of EC from the selected samples of CD patients analyzed carried the fimH30 allele, with a mutation strongly associated with AIEC. Our results show that coinfection with MAP and AIEC is common and persistent in CD, although the high MAP and EC detection in CIR patients suggested that colonization is, at least, partially dependent on increased gut permeability. Nevertheless, facilitative mechanisms between a susceptible host and these two potential human pathogens may allow their implication in CD pathogenesis.

Keywords

Inflammatory bowel disease Mycobacterium avium subsp. paratuberculosis (MAP) Adherent-invasive Escherichia coli (AIEC) IS900, fimH gene 

Notes

Acknowledgments

This work received financial support from the European Union (FEDER funds), through Programa Operacional Factores de Competitividade—COMPETE, and from National Funds (FCT, Fundação para a Ciência e a Tecnologia), through grant number PIC/IC/82802/2007. The authors wish to thank all patients and controls for their participation in this study.

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Nair Nazareth
    • 1
  • Fernando Magro
    • 2
    • 3
    • 4
  • Elisabete Machado
    • 1
    • 5
  • Teresa Gonçalves Ribeiro
    • 1
    • 5
  • António Martinho
    • 1
  • Pedro Rodrigues
    • 1
    • 6
  • Rita Alves
    • 1
  • Gonçalo Nuno Macedo
    • 1
  • Daniela Gracio
    • 2
    • 4
  • Rosa Coelho
    • 3
  • Candida Abreu
    • 7
    • 8
  • Rui Appelberg
    • 6
  • Camila Dias
    • 9
  • Guilherme Macedo
    • 3
  • Tim Bull
    • 10
  • Amélia Sarmento
    • 1
    • 6
  1. 1.FP-ENAS (UFP Energy, Environment and Health Research Unit), CEBIMED (Biomedical Research Centre)University Fernando PessoaPortoPortugal
  2. 2.Institute of Pharmacology and Therapeutics, Faculdade de MedicinaUniversidade do PortoPortoPortugal
  3. 3.Gastroenterology DepartmentCentro Hospitalar São JoãoPortoPortugal
  4. 4.MedInUP -Center for Drug Discovery and Innovative MedicinesUniversidade do PortoPortoPortugal
  5. 5.REQUIMTE, Laboratory of Microbiology, Faculdade de FarmáciaUniversidade do PortoPortoPortugal
  6. 6.Infection and Immunity Unit, IBMC – Instituto de Biologia Molecular e CelularUniversidade do PortoPortoPortugal
  7. 7.Department of Infectious DiseasesCentro Hospitalar S. JoãoPortoPortugal
  8. 8.Nephrology Research and Development UnitFaculdade de Medicina da Universidade do PortoPortoPortugal
  9. 9.Department of Biostatistics and Medical Informatics, Faculdade de MedicinaUniversidade do PortoPortoPortugal
  10. 10.Institute for Infection and ImmunitySt George’s University of LondonLondonUK

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